TeNT Light Chain/Tetanus Toxin Antibody (604023)

Western blot shows recombinant C. tetani TeNT Light Chain. PVDF Membrane was probed with 2 µg/mL of C. tetani TeNT Light Chain Monoclonal Antibody (Catalog # MAB6535) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for TeNT Light Chain at approximately 53 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Ba-Ct
• Applications: WB, IP
• Clone: 604023
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

TeNT Light Chain/Tetanus Toxin Antibody (604023) Summary

• Immunogen: E. coli-derived recombinant C. tetani TeNT Light Chain
  Pro2-Gly430
  Accession # P04958
• Specificity: Detects TeNT Light Chain in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant TeNT Heavy Chain is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TETX
• Purity: Protein A or G purified from hybridoma culture supernatant
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 25 ug/mL
  • Western Blot 2 ug/mL

Reactivity Notes

C. tetani

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A or G purified from hybridoma culture supernatant
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TeNT Light Chain/Tetanus Toxin Antibody (604023)

• TeNT Light Chain
• TeNT-LC
• Tentoxylysin
• Tetanus toxin chain H
• Tetanus toxin chain L
• Tetanus toxin heavy chain
• Tetanus toxin light chain
• tetX

Background

Tetanus toxin, a member of the peptidase M27 family of proteins, is produced by the anaerobic spore-forming bacteria Clostridium tetani as a single-chain polypeptide that is 1315 amino acids (aa) in length.  The protein is subsequently cleaved by an endogenous protease to yield the 52 kDa tetanus toxin light chain (TeNT-LC), which is 457 aa in length, and the 98 kDa tetanus toxin heavy chain that is 858 aa long.  The light and heavy chains are linked by a disulfide bridge and are non-toxic after separation.  Tetanus toxin acts by inhibiting neurotransmitter release.  It binds to peripheral neural synapses, is internalized and moves by retrograde transport up the axon into the spinal cord where it can move between postsynaptic and presynaptic neurons.  It inhibits neurotransmitter release by acting as a zinc endopeptidase that catalyzes the hydrolysis of the ’76-Gln-|-Phe-77’ bond of synaptobrevin-2. 

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TeNT Light Chain/Tetanus Toxin Antibody (MAB6535)(1)

Publication using MAB6535

• JE Cohen, R Wang, RF Shen, WW Wu, JE Keller Comparative pathogenomics of Clostridium tetani PLoS ONE, 2017;12(8):e0182909. 2017 [PMID: 28800585] (Western Blot, Bacteria - Clostridium tetani)

Bioinformatics

• Gene Symbol: TETX
• Uniprot:
  • P04958(C. tetani)