TeNT Light Chain/Tetanus Toxin Antibody (604023)

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Western blot shows recombinant C. tetani TeNT Light Chain. PVDF Membrane was probed with 2 µg/mL of C. tetani TeNT Light Chain Monoclonal Antibody (Catalog # MAB6535) followed by HRP-conjugated Anti-Mouse IgG Secondary ...read more

Product Details

Summary
Reactivity Ba-CtSpecies Glossary
Applications WB, IP
Clone
604023
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Formulation Size Price

TeNT Light Chain/Tetanus Toxin Antibody (604023) Summary

Immunogen
E. coli-derived recombinant C. tetani TeNT Light Chain
Pro2-Gly430
Accession # P04958
Specificity
Detects TeNT Light Chain in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant TeNT Heavy Chain is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
TETX
Purity
Protein A or G purified from hybridoma culture supernatant
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunoprecipitation 25 ug/mL
  • Western Blot 2 ug/mL
Publications
Read Publication using
MAB6535 in the following applications:

Reactivity Notes

C. tetani

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TeNT Light Chain/Tetanus Toxin Antibody (604023)

  • TeNT Light Chain
  • TeNT-LC
  • Tentoxylysin
  • Tetanus toxin chain H
  • Tetanus toxin chain L
  • Tetanus toxin heavy chain
  • Tetanus toxin light chain
  • tetX

Background

Tetanus toxin, a member of the peptidase M27 family of proteins, is produced by the anaerobic spore-forming bacteria Clostridium tetani as a single-chain polypeptide that is 1315 amino acids (aa) in length.  The protein is subsequently cleaved by an endogenous protease to yield the 52 kDa tetanus toxin light chain (TeNT-LC), which is 457 aa in length, and the 98 kDa tetanus toxin heavy chain that is 858 aa long.  The light and heavy chains are linked by a disulfide bridge and are non-toxic after separation.  Tetanus toxin acts by inhibiting neurotransmitter release.  It binds to peripheral neural synapses, is internalized and moves by retrograde transport up the axon into the spinal cord where it can move between postsynaptic and presynaptic neurons.  It inhibits neurotransmitter release by acting as a zinc endopeptidase that catalyzes the hydrolysis of the ’76-Gln-|-Phe-77’ bond of synaptobrevin-2. 

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TeNT Light Chain/Tetanus Toxin Antibody (MAB6535)(1)

We have publications tested in 1 confirmed species: Bacteria - Clostridium tetani.

We have publications tested in 1 application: Western Blot.


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Western Blot
(1)
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Bacteria - Clostridium tetani
(1)
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Product General Protocols

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Video Protocols

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Secondary Antibodies

 

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Additional TeNT Light Chain/Tetanus Toxin Products

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Bioinformatics

Gene Symbol TETX
Uniprot