TeNT Light Chain/Tetanus Toxin Antibody (604023) [Alexa Fluor® 532]

• Reactivity: Ba-Ct
• Applications: WB, IP
• Clone: 604023
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 532

TeNT Light Chain/Tetanus Toxin Antibody (604023) [Alexa Fluor® 532] Summary

• Immunogen: E. coli-derived recombinant C. tetani TeNT Light Chain
  Pro2-Gly430
  Accession # P04958
• Specificity: Detects TeNT Light Chain in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant TeNT Heavy Chain is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TeNT Light Chain/Tetanus Toxin Antibody (604023) [Alexa Fluor® 532]

• TeNT Light Chain
• TeNT-LC
• Tentoxylysin
• Tetanus toxin chain H
• Tetanus toxin chain L
• Tetanus toxin heavy chain
• Tetanus toxin light chain
• tetX

Background

Tetanus toxin, a member of the peptidase M27 family of proteins, is produced by the anaerobic spore-forming bacteria Clostridium tetani as a single-chain polypeptide that is 1315 amino acids (aa) in length.  The protein is subsequently cleaved by an endogenous protease to yield the 52 kDa tetanus toxin light chain (TeNT-LC), which is 457 aa in length, and the 98 kDa tetanus toxin heavy chain that is 858 aa long.  The light and heavy chains are linked by a disulfide bridge and are non-toxic after separation.  Tetanus toxin acts by inhibiting neurotransmitter release.  It binds to peripheral neural synapses, is internalized and moves by retrograde transport up the axon into the spinal cord where it can move between postsynaptic and presynaptic neurons.  It inhibits neurotransmitter release by acting as a zinc endopeptidase that catalyzes the hydrolysis of the ’76-Gln-|-Phe-77’ bond of synaptobrevin-2. 

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • P04958(C. tetani)