TEFM Antibody

Immunocytochemistry/Immunofluorescence: TEFM Antibody [NBP1-82109] - Staining of human cell line U-251 MG shows localization to mitochondria. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: TEFM Antibody [NBP1-82109] - Staining of human skeletal muscle shows no positivity in myocytes as expected.

Immunohistochemistry-Paraffin: TEFM Antibody [NBP1-82109] - Staining of human heart muscle shows weak cytoplasmic/membranous positivity in cardiomyocytes.

Immunohistochemistry-Paraffin: TEFM Antibody [NBP1-82109] - Staining of human lymphoid tissues shows strong cytoplasmic positivity in non-germinal center cells.

Immunohistochemistry-Paraffin: TEFM Antibody [NBP1-82109] - Staining of human prostate shows weak to moderate cytoplasmic positivity in smooth muscle cells.

Product Discontinued

TEFM Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: RRSINIVEHRENFGPFQNLESLMNVPLFKYKSTVQVCNSILCPKTGREKRKSPENRFLRKLLKPDIERERLKAVNSIISIV
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TEFM
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 25690892).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for TEFM Antibody

• C17orf42
• chromosome 17 open reading frame 42
• FLJ22729
• MGC24674
• TEFM
• transcription elongation factor of mitochondria
• transcription elongation factor, mitochondrial

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TEFM Antibody (NBP1-82109)(1)

Publication using NBP1-82109

• Posse V, Shahzad S, Falkenberg M et al. TEFM is a potent stimulator of mitochondrial transcription elongation in vitro. Nucleic Acids Res 2015-03-11 [PMID: 25690892] (WB, Human)

Bioinformatics

• Gene Symbol: TEFM