TBC1D21 Antibody - BSA Free

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Immunohistochemistry-Paraffin: TBC1D21 Antibody [NBP1-92479] - Staining of human placenta shows strong membranous and cytoplasmic positivity in trophoblastic cells.
Immunohistochemistry-Paraffin: TBC1D21 Antibody [NBP1-92479] - Staining of human endometrium shows low expression as expected.
Immunohistochemistry-Paraffin: TBC1D21 Antibody [NBP1-92479] - Staining of human testis shows high expression.
Analysis in control (vector only transfected HEK293T lysate) and TBC1D21 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells).
Orthogonal Strategies: Analysis in human testis and endometrium tissues using Anti-TBC1D21 antibody. Corresponding TBC1D21 RNA-seq data are presented for the same tissues.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Validated by:
       

Orthogonal Strategies

 

Order Details

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Catalog# & Formulation Size Price

TBC1D21 Antibody - BSA Free Summary

Description
Novus Biologicals Rabbit TBC1D21 Antibody - BSA Free (NBP1-92479) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: TLSPENSLSARQSASFILVKRKPPIDKTEWDSFFDESGHLAKSRDFICVNILERGLHPFVRTEAWKFLTGYFSWQSSQDER
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TBC1D21
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
TBC1D21 Protein (NBP1-92479PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (89%), Rat (85%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for TBC1D21 Antibody - BSA Free

  • MGC34741
  • TBC1 domain family member 21
  • TBC1 domain family, member 21

Background

TBC1D21 may act as a GTPase-activating protein for Rab family protein(s)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol TBC1D21