SPTBN4 Antibody (S393-2) - BSA Free

Western Blot: SPTBN4 Antibody (S393-2) [NBP2-59321] - Western Blot analysis of Monkey COS-Beta-4-Spectrin-His showing detection of ~ 200 kDa Beta-4-Spectrin protein using Mouse Anti-Beta-4-Spectrin Monoclonal Antibody, Clone S393-2 (NBP2-59321). Lane 1: MW Ladder. Lane 2: COS-Beta-4-Spectrin-His. Load: 15 ug. Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-Beta-4-Spectrin Monoclonal Antibody (NBP2-59321) at 1:1000 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~ 200 kDa.

Immunocytochemistry/Immunofluorescence: SPTBN4 Antibody (S393-2) [NBP2-59321] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-SPTBN4 Monoclonal Antibody, Clone S393-2 (NBP2-59321). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-SPTBN4 Monoclonal Antibody (NBP2-59321) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) SPTBN4 Antibody (D) Composite.

Immunocytochemistry/Immunofluorescence: SPTBN4 Antibody (S393-2) [NBP2-59321] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-beta 4 Spectrin Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm. Magnification: 60X

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: S393-2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

SPTBN4 Antibody (S393-2) - BSA Free Summary

• Immunogen: Fusion protein amino acids 1621-1832 (C-terminal repeats 14 to 15) of human Beta4-spectrin
• Localization: Cytoplasm , Cytoskeleton , Cytoplasm , Cell Cortex
• Specificity: Detects >200kDa. Does not cross react with other Beta-spectrins
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SPTBN4
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Western Blot 1:1000

Packaging, Storage & Formulations

• Storage: Store at -20C.
• Buffer: PBS (pH 7.4), 50% Glycerol
• Preservative: 0.1% Sodium Azide
• Purity: Protein G purified

Alternate Names for SPTBN4 Antibody (S393-2) - BSA Free

• beta-IV spectrin
• KIAA1642
• QV
• spectrin beta chain, brain 3
• spectrin, beta, non-erythrocytic 4
• spectrin, non-erythroid beta chain 3
• SPNB4
• SPTBN3

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SPTBN4