Sars Spike Protein Antibody

Images

 
Simple Western: Sars Spike Protein Antibody [NB100-56578] - Simple Western lane view shows recombinant SARS-CoV-2 Spike S2 Protein, loaded at 50 ng/mL. A specific band was detected for SARS-CoV-2 Spike S2 Protein at ...read more
Western Blot: Sars Spike Protein Antibody [NB100-56578] - analysis of Spike in (A) transfected and (B) untransfected mouse melanoma cell lysate using this antibody. 05 ug/ml.
Simple Western: Sars Spike Protein Antibody [NB100-56578] - Simple Western lane view shows SARS-CoV-2 lysate, loaded at 1:20 dilution. A specific band was detected for SARS-CoV-2 Spike Protein at approximately 139 kDa ...read more

Product Details

Summary
Reactivity V, VSpecies Glossary
Applications WB, Simple Western, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Sars Spike Protein Antibody Summary

Immunogen
The antibody was developed by immunizing rabbits with a synthetic peptide corresponding to amino acids 1124-1140 (C-QPELDSFKEELDKYFKN) from the S (Spike glycoprotein)(Spike protein S2') for the Human SARS coronavirus (Genbank accession no. NP_828851.1)
Specificity
Full length Spike protein transfected into UM92 cells was used as a positive control and an approximate 139 kDa band was observed. Dot Blot results using recombinant proteins for cross-reactivity testing revealed high reactivity to SARS-CoV-2 Spike Protein, 1000-1200 a.a. (NBP2-90973) and low/no reactivity towards MERS Spike 2 or H1N1 (NBP1-99041).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.05 ug/ml
  • Simple Western 1:10-1:100
  • Immunocytochemistry/Immunofluorescence
Application Notes
Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 16081529).
Control
Recombinant SARS-CoV-2 Spike (1000-1200 a.a.) Protein
Publications
Read Publications using NB100-56578.

Reactivity Notes

Expected species cross reactivity based on immunogen sequence homology: COVID-19 (100%).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS containing 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein G purified

Alternate Names for Sars Spike Protein Antibody

  • COVID-19 Spike
  • E2 glycoprotein
  • E2
  • Human coronavirus spike glycoprotein
  • Peplomer protein
  • S glycoprotein
  • S
  • SARS coronavirus S protein
  • SARS coronavirus Spike Protein
  • SARS CoV S glycoprotein
  • SARS CoV Spike protein
  • SARS CoV
  • SARS-CoV S protein
  • SARS-COV Spike
  • SARSCoV
  • SARS-COV-2 S protein
  • SARS-COV-2 Spike glycoprotein
  • SARSCOV2 Spike protein
  • Severe acute respiratory syndrome spike glycoprotein
  • Severe acute respiratory syndrome virus spike glycoprotein
  • Spike glycoprotein
  • VGL2

Background

It has recently been shown that SARS (severe acute respiratory syndrome) is caused by a human coronavirus. Human coronaviruses are the major cause of upper respiratory tract illness, such as the common cold, in humans. Coronaviruses are positive-stranded RNA viruses, featuring the largest viral RNA genomes known to date (27-31 kb). The first step in coronavirus infection is binding of the viral spike protein, a 139-kDa protein, to certain receptors on host cells. The spike protein is the main surface antigen of the coronavirus. The glycosilated spike protein (as well as the nucleocapsid protein) can be detected in infected cell culture supernatants with antisera from SARS patients.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Publications for Sars Spike Protein Antibody (NB100-56578)(9)

We have publications tested in 2 applications: ICC/IF, WB.


Filter By Application
ICC/IF
(1)
WB
(3)
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Filter By Species
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Showing Publications 1 - 9 of 9.
Publications using NB100-56578 Applications Species
Ujike M, Huang C, Shirato K et al. Two palmitylated cysteine residues of the severe acute respiratory syndrome coronavirus spike (S) protein are critical for S incorporation into virus-like particles, but not for M-S co-localization. J Gen Virol. 2012 Apr [PMID: 22238235]
Bertram S, Glowacka I, Muller MA et al. Cleavage and activation of the severe acute respiratory syndrome coronavirus spike protein by human airway trypsin-like protease. J Virol. 2011 Dec [PMID: 21994442] (WB)

Details:
Product cited: SARS-S Spike protein (IMG-557). WB: 293T cells cotransfected with SARS-S and TTSP expression plasmids, Fig 3. Note: The SARS-S (IMG-557) antibody was transfected validated by WB in 293T cells, Fig 3.
WB
Matsuyama S, Nagata N, Shirato K et al. Efficient activation of the severe acute respiratory syndrome coronavirus spike protein by the transmembrane protease TMPRSS2. J Virol. 2010 Dec [PMID: 20926566]
Watanabe R, Matsuyama S, Shirato K et al. Entry from the cell surface of severe acute respiratory syndrome coronavirus with cleaved S protein as revealed by pseudotype virus bearing cleaved S protein. J Virol. 2008 Dec [PMID: 18786990] (WB)

Details:
WB (HeLa cells), Fig. 2B.
WB
Matsuyama Shutoku, Ujike Makoto, Ishii Koji et al. Enhancement of SARS-CoV infection by proteases. Adv Exp Med Biol. 2006 [PMID: 17037538]
Spruth Martin, Kistner Otfried, Savidis-Dacho Helga et al. A double-inactivated whole virus candidate SARS coronavirus vaccine stimulates neutralising and protective antibody responses. Vaccine. 2006 Jan 30 [PMID: 16214268]
Matsuyama Shutoku, Ujike Makoto, Morikawa Shigeru et al. Protease-mediated enhancement of severe acute respiratory syndrome coronavirus infection. Proc Natl Acad Sci U S A. 2005 Aug 30 [PMID: 16116101] (WB) WB
Simmons G, Gosalia DN, Rennekamp AJ et al. Inhibitors of cathepsin L prevent severe acute respiratory syndrome coronavirus entry. Proc Natl Acad Sci U S A. 2005 Aug 16 [PMID: 16081529] (ICC/IF) ICC/IF
Fukushi S, Mizutani T, Saijo M et al. Vesicular stomatitis virus pseudotyped with severe acute respiratory syndrome coronavirus spike protein. J Gen Virol. 2005 Aug [PMID: 16033974]

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Sars Spike Protein Antibody (NB100-56578). (Showing 1 - 3 of 3 FAQs).

  1. Do you know if Sars Spike Protein Antibody (16F1071) will cross react with SARS-2?
    • NBP2-24746 has no similarity unfortunately. However, NBP2-24808, NBP2-24942, and NB100-56578 have 100% sequence homology with the SARS-CoV-2 Spike Protein.
  2. I am looking for a primary antibody to sars spike protein to the new COVID-19.
    • NBP1-28850, NB100-56578, NBP2-24808, NBP2-24942 have 100% homology to the new SARS-CoV-2 virus.
  3. Hello can you help me to identify detection antibody for COvid-19 spike protein? NB100-56578 is for spike of the Covid-19 not for the original SARS virus correct?
    • NB100-56578 was originally developed to the original SARS virus, but the homology of the target sequence of this antibody to the spike protein for COVID-19 is identical, so we can guarantee that it will work for COVID-19 detection.
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