Simple Western: Sars Spike Protein Antibody [NB100-56578] - Simple Western lane view shows recombinant SARS-CoV-2 Spike S2 Protein, loaded at 50 ng/mL. A specific band was detected for SARS-CoV-2 Spike S2 Protein at ...read more
Western Blot: Sars Spike Protein Antibody [NB100-56578] - analysis of Spike in (A) transfected and (B) untransfected mouse melanoma cell lysate using this antibody. 05 ug/ml.
Simple Western: Sars Spike Protein Antibody [NB100-56578] - Simple Western lane view shows SARS-CoV-2 lysate, loaded at 1:20 dilution. A specific band was detected for SARS-CoV-2 Spike Protein at approximately 139 kDa ...read more
The antibody was developed by immunizing rabbits with a synthetic peptide corresponding to amino acids 1124-1140 (C-QPELDSFKEELDKYFKN) from the S (Spike glycoprotein)(Spike protein S2') for the Human SARS coronavirus (Genbank accession no. NP_828851.1)
Full length Spike protein transfected into UM92 cells was used as a positive control and an approximate 139 kDa band was observed. Dot Blot results using recombinant proteins for cross-reactivity testing revealed high reactivity to SARS-CoV-2 Spike Protein, 1000-1200 a.a. (NBP2-90973) and low/no reactivity towards MERS Spike 2 or H1N1 (NBP1-99041).
Protein G purified
Test in a species/application not listed above to receive a full credit towards a future purchase.
Expected species cross reactivity based on immunogen sequence homology: COVID-19 (100%).
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS containing 0.05% BSA
0.05% Sodium Azide
Protein G purified
Alternate Names for Sars Spike Protein Antibody
Human coronavirus spike glycoprotein
SARS coronavirus S protein
SARS coronavirus Spike Protein
SARS CoV S glycoprotein
SARS CoV Spike protein
SARS-CoV S protein
SARS-COV-2 S protein
SARS-COV-2 Spike glycoprotein
SARSCOV2 Spike protein
Severe acute respiratory syndrome spike glycoprotein
Severe acute respiratory syndrome virus spike glycoprotein
It has recently been shown that SARS (severe acute respiratory syndrome) is caused by a human coronavirus. Human coronaviruses are the major cause of upper respiratory tract illness, such as the common cold, in humans. Coronaviruses are positive-stranded RNA viruses, featuring the largest viral RNA genomes known to date (27-31 kb). The first step in coronavirus infection is binding of the viral spike protein, a 139-kDa protein, to certain receptors on host cells. The spike protein is the main surface antigen of the coronavirus. The glycosilated spike protein (as well as the nucleocapsid protein) can be detected in infected cell culture supernatants with antisera from SARS patients.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Publications for Sars Spike Protein Antibody (NB100-56578)(9)
We have publications tested in 2 applications: ICC/IF, WB.
FAQs for Sars Spike Protein Antibody (NB100-56578). (Showing 1 - 3 of 3 FAQs).
Do you know if Sars Spike Protein Antibody (16F1071) will cross react with SARS-2?
NBP2-24746 has no similarity unfortunately. However, NBP2-24808, NBP2-24942, and NB100-56578 have 100% sequence homology with the SARS-CoV-2 Spike Protein.
I am looking for a primary antibody to sars spike protein to the new COVID-19.
NBP1-28850, NB100-56578, NBP2-24808, NBP2-24942 have 100% homology to the new SARS-CoV-2 virus.
Hello can you help me to identify detection antibody for COvid-19 spike protein? NB100-56578 is for spike of the Covid-19 not for the original SARS virus correct?
NB100-56578 was originally developed to the original SARS virus, but the homology of the target sequence of this antibody to the spike protein for COVID-19 is identical, so we can guarantee that it will work for COVID-19 detection.
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PRODUCT AVAILABILITY: Update Regarding the Evolving COVID-19 Situation
Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally.