SARS Spike Protein Antibody


Simple Western: Sars Spike Protein Antibody [NB100-56578] - Simple Western lane view shows recombinant SARS-CoV-2 Spike S2 Protein, loaded at 50 ng/mL. A specific band was detected for SARS-CoV-2 Spike S2 Protein at more
Western Blot: Sars Spike Protein Antibody [NB100-56578] - SARS-CoV-2 spike overexpressed 293T cell lysate. Antibody dilution: 1:3000. Two bands for full length spike and cleaved S2 are shown, respectively. Western blot more
Western Blot: SARS Spike Protein Antibody [NB100-56578] - Western blot shows SARS-CoV-1 Spike (Catalog # 10581-CV), SARS CoV-2 Spike (Catalog # 10586-CV), SArS-CoV-2 Spike S2 Subunit (Catalog # 10584-CV). Membrane was more
Western Blot: Sars Spike Protein Antibody [NB100-56578] - Western blot analysis of SARS Spike Protein in (A) transfected and (B) untransfected mouse melanoma cell lysate.
Western Blot: Sars Spike Protein Antibody [NB100-56578] - Western blot shows recombinant protein SARS-CoV-2 Spike S2 Subunit. Membrane was probed with 0.5 ug/mL of Rabbit Anti-SARS Spike Protein Polyclonal Antibody more
Simple Western: Sars Spike Protein Antibody [NB100-56578] - Simple Western lane view shows SARS-CoV-2 lysate, loaded at 1:20 dilution. SARS-CoV-2 Spike Protein was detected using 6.25 ug/mL of Rabbit Anti-SARS-CoV Spike more

Product Details

Reactivity V, VSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC
1.0 mg/ml

SARS Spike Protein Antibody Summary

The antibody was developed by immunizing rabbits with a synthetic peptide corresponding to amino acids 1124-1140 (C-GNCDVVIGIVNNTVYDP) from the S (Spike glycoprotein)(Spike protein S2') for the Human SARS coronavirus (Genbank accession no. YP_009724390)
Full length Spike protein transfected into UM92 cells was used as a positive control and an approximate 139 kDa band was observed. Dot Blot results using recombinant proteins for cross-reactivity testing revealed high reactivity to SARS-CoV-2 Spike Protein, 1000-1200 a.a. (NBP2-90973) and low/no reactivity towards MERS Spike 2 or H1N1 (NBP1-99041).
Immunogen affinity purified
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Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Immunocytochemistry/Immunofluorescence reported in scientific literature (PMID 16081529)
  • Immunohistochemistry reported in scientific literature (PMID 34357881)
  • Simple Western 1:40 - 1:160
  • Western Blot 0.05 - 1.0 ug/mL
Recombinant Virus SARS-CoV-2 Spike (1000-1200 a.a.) His (C-Term) Protein
Reviewed Applications
Read 1 Review rated 5
NB100-56578 in the following applications:

Read Publications using
NB100-56578 in the following applications:

  • 2 publications
  • 2 publications
  • SW
    1 publication
  • WB
    12 publications

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02% Sodium Azide
1.0 mg/ml
Immunogen affinity purified

Alternate Names for SARS Spike Protein Antibody

  • COVID-19 Spike
  • E2 glycoprotein
  • E2
  • Human coronavirus spike glycoprotein
  • Peplomer protein
  • S glycoprotein
  • S
  • SARS coronavirus S protein
  • SARS coronavirus Spike Protein
  • SARS CoV S glycoprotein
  • SARS CoV Spike protein
  • SARS CoV
  • SARS-CoV S protein
  • SARS-COV Spike
  • SARS-COV-2 S protein
  • SARS-COV-2 Spike glycoprotein
  • SARSCOV2 Spike protein
  • Severe acute respiratory syndrome spike glycoprotein
  • Severe acute respiratory syndrome virus spike glycoprotein
  • Spike glycoprotein
  • VGL2


It has recently been shown that SARS (severe acute respiratory syndrome) is caused by a human coronavirus. Human coronaviruses are the major cause of upper respiratory tract illness, such as the common cold, in humans. Coronaviruses are positive-stranded RNA viruses, featuring the largest viral RNA genomes known to date (27-31 kb). The first step in coronavirus infection is binding of the viral spike protein, a 139-kDa protein, to certain receptors on host cells. The spike protein is the main surface antigen of the coronavirus. The glycosilated spike protein (as well as the nucleocapsid protein) can be detected in infected cell culture supernatants with antisera from SARS patients.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Publications for SARS Spike Protein Antibody (NB100-56578)(25)

We have publications tested in 2 confirmed species: Human, SARS-CoV-2.

We have publications tested in 4 applications: ICC/IF, IF/IHC, SW, WB.

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Showing Publications 1 - 10 of 25. Show All 25 Publications.
Publications using NB100-56578 Applications Species
Meng W, Guo S, Cao S et al. Development and characterization of a new monoclonal antibody against SARS-CoV-2 NSP12 (RdRp) Journal of medical virology 2022-10-21 [PMID: 36271490]
Hornich BF, Grobkopf AK, Schlagowski S et al. SARS-CoV-2 and SARS-CoV Spike-Mediated Cell-Cell Fusion Differ in Their Requirements for Receptor Expression and Proteolytic Activation Journal of Virology 2021-05-12 [PMID: 33608407] (WB) WB
Peacock TP, Brown JC, Zhou J et al. The SARS-CoV-2 variant, Omicron, shows rapid replication in human primary nasal epithelial cultures and efficiently uses the endosomal route of entry. bioRxiv 2022-01-01 (WB) WB
Tan CCS, Trew J, Peacock TP et al. Genomic screening of 16 UK native bat species through conservationist networks uncovers coronaviruses with zoonotic potential Nature communications 2023-06-27 [PMID: 37369644] (WB) WB
Vu MN, Morris DR, Alvarado RE et al. Loss-of-function mutation in Omicron variants reduces spike protein expression and attenuates SARS-CoV-2 infection bioRxiv : the preprint server for biology 2023-04-18 [PMID: 37131784] (WB) WB
Warner BM, Santry LA, Leacy A Et al. Intranasal vaccination with a Newcastle disease virus-vectored vaccine protects hamsters from SARS-CoV-2 infection and disease iScience 2021-11-19 [PMID: 34632328] (WB) WB
GarcIa-Dorival I, Cuesta-Geijo MA, Barrado-Gil L Et al. Identification of Niemann-Pick C1 protein as a potential novel SARS-CoV-2 intracellular target Antiviral research 2021-08-24 [PMID: 34450201] (WB, SARS-CoV-2) WB SARS-CoV-2
Schaller MA, Sharma Y, Dupee Z Et al. Ex vivo SARS-CoV-2 infection of human lung reveals heterogeneous host defense and therapeutic responses JCI insight 2021-08-06 [PMID: 34357881] (IF/IHC, SARS-CoV-2) IF/IHC SARS-CoV-2
Meng B, Kemp SA, Papa G et al. Recurrent emergence of SARS-CoV-2 spike deletion H69/V70 and its role in the variant of concern lineage B.1.1.7 Cell 2021-06-29 [PMID: 34166617]
Show All 25 Publications.

Review for SARS Spike Protein Antibody (NB100-56578) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-56578:
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Images Ratings Applications Species Date Details
Western Blot SARS Spike Protein NB100-56578
reviewed by:
Shaobo Wang
WB Human 10/02/2020


ApplicationWestern Blot
Sample TestedSARS-CoV-2 spike transfected 293T cell lysate

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SARS Spike Protein Antibody (NB100-56578). (Showing 1 - 4 of 4 FAQs).

  1. Do you know if Sars Spike Protein Antibody (16F1071) will cross react with SARS-2?
    • NBP2-24746 has no similarity unfortunately. However, NBP2-24808, NBP2-24942, and NB100-56578 have 100% sequence homology with the SARS-CoV-2 Spike Protein.
  2. I am looking for a primary antibody to sars spike protein to the new COVID-19.
    • NBP1-28850, NB100-56578, NBP2-24808, NBP2-24942 have 100% homology to the new SARS-CoV-2 virus.
  3. Hello can you help me to identify detection antibody for COvid-19 spike protein? NB100-56578 is for spike of the Covid-19 not for the original SARS virus correct?
    • NB100-56578 was originally developed to the original SARS virus, but the homology of the target sequence of this antibody to the spike protein for COVID-19 is identical, so we can guarantee that it will work for COVID-19 detection.
  4. The spec sheet says that NB100-56578 species cross reactivity 100% wonder what that means…
    • It means we have blasted homology between our immunogens and COVID19, and this one is 100% homologous. However, we do not have COVID19 samples to validate with, so it is predicted to work on COVID19.

Control Lysate(s)

Secondary Antibodies


Isotype Controls

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Recent Reviews


Shaobo Wang
Application: WB
Species: Human