SARS Nucleocapsid Protein Antibody


Simple Western: SARS Nucleocapsid Protein Antibody [NB100-56683] - Simple Western lane view shows recombinant SARS-CoV-2 Nucleocapsid Protein (Catalog # 10474-CV), loaded at 20 ng/mL. A specific band was detected for more
Orthogonal Strategies: Dual RNAscope ISH-IHC: SARS Nucleocapsid Protein Antibody [NB100-56683] - Formalin-fixed paraffin-embedded tissue sections of SARS-CoV-2 infected human lung tissue were probed for more
Simple Western: SARS Nucleocapsid Protein Antibody [NB100-56683] - Simple Western lane view shows lysates of SARS-CoV-2 (1:50), MERS (1:100), OC43 (1:100), and 229E (1:100). A specific band was detected for SARS-CoV-2 more
Western Blot: SARS Nucleocapsid Protein Antibody [NB100-56683] - Analysis of SARS Nucleocapsid in (A) untransfected mouse melanoma cell lysate and (B) transfected cell lysate using this antibody at a 1:2000 dilution.

Product Details

Reactivity V, VSpecies Glossary
Applications WB, Simple Western, ELISA, ICC/IF, IHC, Dual ISH-IHC
1 mg/ml
Validated by:

Orthogonal Strategies


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SARS Nucleocapsid Protein Antibody Summary

The antibody was developed by immunizing rabbits with a synthetic peptide corresponding to amino acids 354-370 (NKHIDAYKTFPPTEPKK-C) from the N (SARS Nucleocapsid) for the Human SARS coronavirus (Genbank accession no. YP_009724397.2)
The was tested on a human cell line transfected with full-length SARS Nucleocapsid cDNA with a predicted molecular weight of 46 kDa.
Protein G purified
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Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Dual RNAscope ISH-IHC
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence reported in scientific literature (PMID 16014910)
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Simple Western 1:50
  • Western Blot 1:100-1:2000
Read Publications using
NB100-56683 in the following applications:

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.05% Sodium Azide
1 mg/ml
Protein G purified

Alternate Names for SARS Nucleocapsid Protein Antibody

  • COVID-19 nucleocapsid protein
  • N protein
  • N structural protein
  • N
  • NC
  • Nucleocapsid protein
  • Nucleoprotein
  • Protein N
  • SARS coronavirus N protein
  • SARS coronavirus nucleocapsid protein
  • SARS CoV N protein
  • SARS CoV nucleocapsid protein
  • SARS CoV
  • SARS N protein
  • SARS Nucleoprotein
  • SARSCoV N protein
  • SARSCoV nucleocapsid protein
  • SARSCOV2 N protein
  • SARS-COV-2 nucleocapsid protein
  • SARS-COV-2 Nucleoprotein
  • Severe acute respiratory syndrome


It has recently been shown that SARS is caused by a human coronavirus. Human coronaviruses are the major cause of upper respiratory tract illness in humans, such as the common cold. Coronaviruses are positive-stranded RNA viruses, featuring the largest viral RNA genomes known to date (27-31 kb). The first step in coronavirus infection is binding of the viral spike protein, a 139-kDa protein, to certain receptors on host cells. The spike protein is the main surface antigen of the coronavirus. The most prominent protein in the culture supernatants infected with SARS virus is a 46 kDa nucleocapsid protein. This suggests that the nucleocapsid protein is a major immunogen that may be useful for early diagnostics.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Publications for SARS Nucleocapsid Protein Antibody (NB100-56683)(19)

We have publications tested in 3 confirmed species: Human, Hamster, SARS-CoV-2.

We have publications tested in 4 applications: ICC/IF, IF/IHC, IHC-P, WB.

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Showing Publications 1 - 10 of 19. Show All 19 Publications.
Publications using NB100-56683 Applications Species
Gibson SA Establishment of a Transgenic Human Angiotensin Converting Enzyme-2 Hamster Infection Model for the Evaluation of Therapeutics Against Severe Acute Respiratory Syndrome Coronavirus 2 Thesis 2023-01-01 (IHC-P, Hamster) IHC-P Hamster
Chang J, Grimley S, Tran B et al. Uncovering strain- and age- dependent differences in innate immune response to SARS-CoV-2 infection in nasal epithelia using combined short and long-read scRNA-seq bioRxiv 2023-03-09 (ICC/IF) ICC/IF
Basolo A, Poma AM, Macerola E et al. AUTOPSY STUDY OF TESTICLES IN COVID-19: UPREGULATION OF IMMUNE-RELATED GENES AND DOWNREGULATION OF TESTIS-SPECIFIC GENES The Journal of clinical endocrinology and metabolism 2022-10-19 [PMID: 36260523] (IHC-P, Human) IHC-P Human
Planes R, Pinilla M, Santoni K et al. Human NLRP1 is a sensor of pathogenic coronavirus 3CL proteases in lung epithelial cells Molecular cell 2022-05-16 [PMID: 35594856] (WB, SARS-CoV-2) WB SARS-CoV-2
Basolo A, Poma AM, Bonuccelli D et al. Adipose tissue in COVID-19: detection of SARS-CoV-2 in adipocytes and activation of the interferon-alpha response Journal of endocrinological investigation 2022-02-15 [PMID: 35169984] (IF/IHC, SARS-CoV-2) IF/IHC SARS-CoV-2
Gerber PP, Duncan LM, Greenwood EJ et al. A protease-activatable luminescent biosensor and reporter cell line for authentic SARS-CoV-2 infection PLoS pathogens 2022-02-01 [PMID: 35143592]
Bestion E, Zandi K, Belouzard S et al. GNS561 Exhibits Potent Antiviral Activity against SARS-CoV-2 through Autophagy Inhibition Viruses 2022-01-12 [PMID: 35062337] (WB) WB
Cate D, Hsieh H, Glukhova V et al. Antibody Screening Results for Anti-Nucleocapsid Antibodies Towards the Development of a SARS-CoV-2 Nucleocapsid Protein Antigen Detecting Lateral Flow Assay ChemRxiv 2020-07-27 [PMID: 34608447]
Poma AM, Basolo A, Bonuccelli D et al. Activation of Type I and Type II Interferon signaling in SARS-CoV-2-positive thyroid tissue of patients dying from COVID-19 Thyroid : official journal of the American Thyroid Association 2021-09-19 [PMID: 34541878]
Tomchaney M, Contoli M, Mayo J Et al. Paradoxical effects of cigarette smoke and COPD on SARS-CoV-2 infection and disease BMC pulmonary medicine 2021-08-23 [PMID: 34425811] (ICC/IF) ICC/IF
Show All 19 Publications.

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Product General Protocols

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WB Video Protocol
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Gene Symbol N