SARS Nucleocapsid Protein Antibody - BSA Free

Simple Western: SARS Nucleocapsid Protein Antibody [NB100-56683] - Simple Western lane view shows recombinant SARS-CoV-2 Nucleocapsid Protein (Catalog # 10474-CV), loaded at 20 ng/mL. A specific band was detected for SARS-CoV-2 Nucleocapsid Protein at approximately 60 kDa (as indicated) using a serial dilution of Rabbit Anti-SARS-CoV Nucleocapsid Protein Polyclonal Antibody (Catalog # NB100-56683) followed by incubation with HRP-conjugated Anti-Goat IgG Secondary Antibody. This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Dual RNAscope ISH-IHC: SARS Nucleocapsid Protein Antibody [NB100-56683] - Formalin-fixed paraffin-embedded tissue sections of SARS-CoV-2 infected human lung tissue were probed for SARS-CoV-2 viral RNA (ACD anti-sense specific probe v-nCoV2019-S [848561]); Fast Red chromogen, ACD [322360]). Adjacent tissue section was processed for immunohistochemistry using rabbit polyclonal anti-SARS Nucleocapsid Antibody [NB100-56683] at 15ug/mL with 1 hr incubation at 25 degrees Celsius followed by incubation with anti-rabbit IgG VisUCyte HRP Polymer Antibody [VC003] and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to SARS-CoV-2 infected cells.

Simple Western: SARS Nucleocapsid Protein Antibody [NB100-56683] - Simple Western lane view shows lysates of SARS-CoV-2 (1:50), MERS (1:100), OC43 (1:100), and 229E (1:100). A specific band was detected for SARS-CoV-2 Nucleocapsid Protein at approximately 60 kDa (as indicated) only in the SARS-CoV-2 lysate using 25 ug/mL of Rabbit Anti-SARS-CoV Nucleocapsid Protein Polyclonal Antibody (Catalog # NB100-56683) followed by incubation with HRP-conjugated Anti-Goat IgG Secondary Antibody. This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Note: some reactivity observed with FL Std 230. SARS-CoV-2 lysate courtesy of University of Maryland.

Western Blot: SARS Nucleocapsid Protein Antibody [NB100-56683] - Analysis of SARS Nucleocapsid in (A) untransfected mouse melanoma cell lysate and (B) transfected cell lysate using this antibody at a 1:2000 dilution.

• Reactivity: V, V
• Applications: WB, Simple Western, ELISA, ICC/IF, IHC, Dual ISH-IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

SARS Nucleocapsid Protein Antibody - BSA Free Summary

• Immunogen: The antibody was developed by immunizing rabbits with a synthetic peptide corresponding to amino acids 354-370 (NKHIDAYKTFPPTEPKK-C) from the N (SARS Nucleocapsid) for the Human SARS coronavirus (Genbank accession no. YP_009724397.2)
• Specificity: The was tested on a human cell line transfected with full-length SARS Nucleocapsid cDNA with a predicted molecular weight of 46 kDa.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: N
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Dual RNAscope ISH-IHC
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence reported in scientific literature (PMID 16014910)
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Simple Western 1:50
  • Western Blot 1:100-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for SARS Nucleocapsid Protein Antibody - BSA Free

• COVID-19 nucleocapsid protein
• N protein
• N structural protein
• N
• NC
• Nucleocapsid protein
• Nucleoprotein
• Protein N
• SARS coronavirus N protein
• SARS coronavirus nucleocapsid protein
• SARS CoV N protein
• SARS CoV nucleocapsid protein
• SARS CoV
• SARS N protein
• SARS Nucleoprotein
• SARSCoV N protein
• SARSCoV nucleocapsid protein
• SARSCoV
• SARSCOV2 N protein
• SARS-COV-2 nucleocapsid protein
• SARS-COV-2 Nucleoprotein
• Severe acute respiratory syndrome

Background

It has recently been shown that SARS is caused by a human coronavirus. Human coronaviruses are the major cause of upper respiratory tract illness in humans, such as the common cold. Coronaviruses are positive-stranded RNA viruses, featuring the largest viral RNA genomes known to date (27-31 kb). The first step in coronavirus infection is binding of the viral spike protein, a 139-kDa protein, to certain receptors on host cells. The spike protein is the main surface antigen of the coronavirus. The most prominent protein in the culture supernatants infected with SARS virus is a 46 kDa nucleocapsid protein. This suggests that the nucleocapsid protein is a major immunogen that may be useful for early diagnostics.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SARS Nucleocapsid Protein Antibody (NB100-56683)(23)

Publications using NB100-56683

• Gibson SA Establishment of a Transgenic Human Angiotensin Converting Enzyme-2 Hamster Infection Model for the Evaluation of Therapeutics Against Severe Acute Respiratory Syndrome Coronavirus 2 Thesis 2023-01-01 (IHC-P, Hamster)
• Chang J, Grimley S, Tran B et al. Uncovering strain- and age- dependent differences in innate immune response to SARS-CoV-2 infection in nasal epithelia using combined short and long-read scRNA-seq bioRxiv 2023-03-09 (ICC/IF)
• Basolo A, Poma AM, Macerola E et al. AUTOPSY STUDY OF TESTICLES IN COVID-19: UPREGULATION OF IMMUNE-RELATED GENES AND DOWNREGULATION OF TESTIS-SPECIFIC GENES The Journal of clinical endocrinology and metabolism 2022-10-19 [PMID: 36260523] (IHC-P, Human)
• Planes R, Pinilla M, Santoni K et al. Human NLRP1 is a sensor of pathogenic coronavirus 3CL proteases in lung epithelial cells Molecular cell 2022-05-16 [PMID: 35594856] (WB, SARS-CoV-2)
• Poma AM, Proietti A, Macerola E, Bonuccelli D et Al. Suppression of Pituitary Hormone Genes in Subjects Who Died From COVID-19 Independently of Virus Detection in the Gland J Clin Endocrinol Metab 2022-05-14 [PMID: 35567590]
• Basolo A, Poma AM, Bonuccelli D et al. Adipose tissue in COVID-19: detection of SARS-CoV-2 in adipocytes and activation of the interferon-alpha response Journal of endocrinological investigation 2022-02-15 [PMID: 35169984] (IF/IHC, SARS-CoV-2)
• Gerber PP, Duncan LM, Greenwood EJ et al. A protease-activatable luminescent biosensor and reporter cell line for authentic SARS-CoV-2 infection PLoS pathogens 2022-02-01 [PMID: 35143592]
• Bestion E, Zandi K, Belouzard S et al. GNS561 Exhibits Potent Antiviral Activity against SARS-CoV-2 through Autophagy Inhibition Viruses 2022-01-12 [PMID: 35062337] (WB)
• Cate D, Hsieh H, Glukhova V et al. Antibody Screening Results for Anti-Nucleocapsid Antibodies Towards the Development of a SARS-CoV-2 Nucleocapsid Protein Antigen Detecting Lateral Flow Assay ChemRxiv 2020-07-27 [PMID: 34608447]
• Poma AM, Basolo A, Bonuccelli D et al. Activation of Type I and Type II Interferon signaling in SARS-CoV-2-positive thyroid tissue of patients dying from COVID-19 Thyroid : official journal of the American Thyroid Association 2021-09-19 [PMID: 34541878]
• Tomchaney M, Contoli M, Mayo J Et al. Paradoxical effects of cigarette smoke and COPD on SARS-CoV-2 infection and disease BMC pulmonary medicine 2021-08-23 [PMID: 34425811] (ICC/IF)
• Schaller MA, Sharma Y, Dupee Z Et al. Ex vivo SARS-CoV-2 infection of human lung reveals heterogeneous host defense and therapeutic responses JCI insight 2021-08-06 [PMID: 34357881] (IF/IHC, SARS-CoV-2)
• Poma Am, Bonuccelli D, Giannini R Et Al. COVID-19 autopsy cases: detection of virus in endocrine tissues Journal of endocrinological investigation 2021-06-30 [PMID: 34191258]
• Meng B, Kemp SA, Papa G et al. Recurrent emergence of SARS-CoV-2 spike deletion H69/V70 and its role in the variant of concern lineage B.1.1.7 Cell 2021-06-29 [PMID: 34166617]
• Chapman AP, Tang X, Lee JR et al. Rapid development of neutralizing and diagnostic SARS-COV-2 mouse monoclonal antibodies Scientific reports 2021-05-06 [PMID: 33958613] (IHC-P)
• Cross RW, Prasad AN, Borisevich V et al. Use of convalescent serum reduces severity of COVID-19 in nonhuman primates Cell Reports 2021-03-01 [PMID: 33662255] (Immunocytochemistry/ Immunofluorescence)
• Cross RW, Prasad AN, Borisevich V et al. Use of convalescent serum reduces severity of COVID-19 in nonhuman primates Cell reports Mar 9 2021 [PMID: 33662255] (IHC, Human, Hamster)
• Rebendenne A, Valad�o ALC, Tauziet M et al. SARS-CoV-2 triggers an MDA-5-dependent interferon response which is unable to control replication in lung epithelial cells Journal of Virology 2021-03-25 [PMID: 33514628] (Immunocytochemistry/ Immunofluorescence)
• Shulla A, Heald-Sargent T, Subramanya G et al. A transmembrane serine protease is linked to the severe acute respiratory syndrome coronavirus receptor and activates virus entry. J Virol. 2011-01-01 [PMID: 21068237] (WB)
  
  Details:
  WB: SARS-Nucleocapsid protein (IMG-549) & SARS-Spike protein (IMG-541). 293T cells harvested 24 hours post infection with the SARS Coronavirus, Fig 9B.
• Devaraj SG, Wang N, Chen Z et al. Regulation of IRF-3-dependent innate immunity by the papain-like protease domain of the severe acute respiratory syndrome coronavirus. J Biol Chem. 2007-11-02 [PMID: 17761676]
• Wathelet MG, Orr M, Frieman MB, Baric RS. Severe acute respiratory syndrome coronavirus evades antiviral signaling: role of nsp1 and rational design of an attenuated strain. J Virol. 2007-11-01 [PMID: 17715225]
• Gupta V, Tabiin TM, Sun K et al. SARS coronavirus nucleocapsid immunodominant T-cell epitope cluster is common to both exogenous recombinant and endogenous DNA-encoded immunogens. Virology. 2006-03-30 [PMID: 16387339] (WB)
  
  Details:
  Confocal, EM
• Tseng CT, Tseng J, Perrone L et al. Apical entry and release of severe acute respiratory syndrome-associated coronavirus in polarized Calu-3 lung epithelial cells. J Virol. 2005-08-01 [PMID: 16014910] (ICC/IF)

Bioinformatics

• Gene Symbol: N
• Uniprot:
  • NP_828858.1()
  • NP_828858.1()
  • P59595(Viral)