SARS Nucleocapsid Protein Antibody


Simple Western: SARS Nucleocapsid Protein Antibody [NB100-56683] - Simple Western lane view shows recombinant SARS-CoV-2 Nucleocapsid Protein (Catalog # 10474-CV), loaded at 20 ng/mL. A specific band was detected for more
Orthogonal Strategies: Dual RNAscope ISH-IHC: SARS Nucleocapsid Protein Antibody [NB100-56683] - Formalin-fixed paraffin-embedded tissue sections of SARS-CoV-2 infected human lung tissue were probed for more
Simple Western: SARS Nucleocapsid Protein Antibody [NB100-56683] - Simple Western lane view shows lysates of SARS-CoV-2 (1:50), MERS (1:100), OC43 (1:100), and 229E (1:100). A specific band was detected for SARS-CoV-2 more
Western Blot: SARS Nucleocapsid Protein Antibody [NB100-56683] - Analysis of SARS Nucleocapsid in (A) untransfected mouse melanoma cell lysate and (B) transfected cell lysate using this antibody at a 1:2000 dilution.

Product Details

Reactivity V, VSpecies Glossary
Applications WB, Simple Western, ELISA, ICC/IF, IHC, IHC-P, Dual ISH-IHC
1 mg/ml
Validated by:

Orthogonal Strategies


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SARS Nucleocapsid Protein Antibody Summary

The antibody was developed by immunizing rabbits with a synthetic peptide corresponding to amino acids 354-370 (NKHIDAYKTFPPTEPKK-C) from the N (SARS Nucleocapsid) for the Human SARS coronavirus (Genbank accession no. YP_009724397.2)
The was tested on a human cell line transfected with full-length SARS Nucleocapsid cDNA with a predicted molecular weight of 46 kDa.
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Dual RNAscope ISH-IHC
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Simple Western 1:50
  • Western Blot 1:100-1:2000
Application Notes
Use in ICC/IF reported in scientific literature (PMID: 16014910).
Read Publications using
NB100-56683 in the following applications:

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: COVID-19 (100%)

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.05% Sodium Azide
1 mg/ml
Protein G purified

Alternate Names for SARS Nucleocapsid Protein Antibody

  • COVID-19 nucleocapsid protein
  • N protein
  • N structural protein
  • N
  • NC
  • Nucleocapsid protein
  • Nucleoprotein
  • Protein N
  • SARS coronavirus N protein
  • SARS coronavirus nucleocapsid protein
  • SARS CoV N protein
  • SARS CoV nucleocapsid protein
  • SARS CoV
  • SARS N protein
  • SARS Nucleoprotein
  • SARSCoV N protein
  • SARSCoV nucleocapsid protein
  • SARSCOV2 N protein
  • SARS-COV-2 nucleocapsid protein
  • SARS-COV-2 Nucleoprotein
  • Severe acute respiratory syndrome


It has recently been shown that SARS is caused by a human coronavirus. Human coronaviruses are the major cause of upper respiratory tract illness in humans, such as the common cold. Coronaviruses are positive-stranded RNA viruses, featuring the largest viral RNA genomes known to date (27-31 kb). The first step in coronavirus infection is binding of the viral spike protein, a 139-kDa protein, to certain receptors on host cells. The spike protein is the main surface antigen of the coronavirus. The most prominent protein in the culture supernatants infected with SARS virus is a 46 kDa nucleocapsid protein. This suggests that the nucleocapsid protein is a major immunogen that may be useful for early diagnostics.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Publications for SARS Nucleocapsid Protein Antibody (NB100-56683)(11)

We have publications tested in 1 confirmed species: SARS-CoV-2.

We have publications tested in 4 applications: ICC/IF, IHC, IHC-P, WB.

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Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using NB100-56683 Applications Species
Tomchaney M, Contoli M, Mayo J Et al. Paradoxical effects of cigarette smoke and COPD on SARS-CoV-2 infection and disease BMC pulmonary medicine Aug 23 2021 [PMID: 34425811] (ICC/IF) ICC/IF
Schaller MA, Sharma Y, Dupee Z Et al. Ex vivo SARS-CoV-2 infection of human lung reveals heterogeneous host defense and therapeutic responses JCI insight Aug 6 2021 [PMID: 34357881] (IHC, SARS-CoV-2) IHC SARS-CoV-2
Poma Am, Bonuccelli D, Giannini R Et Al. COVID-19 autopsy cases: detection of virus in endocrine tissues Journal of endocrinological investigation 2021 Jun 30 [PMID: 34191258]
Meng B, Kemp SA, Papa G et al. Recurrent emergence of SARS-CoV-2 spike deletion H69/V70 and its role in the variant of concern lineage B.1.1.7 Cell Jun 29 2021 [PMID: 34166617]
Shulla A, Heald-Sargent T, Subramanya G et al. A transmembrane serine protease is linked to the severe acute respiratory syndrome coronavirus receptor and activates virus entry. J Virol. 2011 Jan [PMID: 21068237] (WB)

WB: SARS-Nucleocapsid protein (IMG-549) & SARS-Spike protein (IMG-541). 293T cells harvested 24 hours post infection with the SARS Coronavirus, Fig 9B.
Devaraj SG, Wang N, Chen Z et al. Regulation of IRF-3-dependent innate immunity by the papain-like protease domain of the severe acute respiratory syndrome coronavirus. J Biol Chem. 2007 Nov 2 [PMID: 17761676]
Wathelet MG, Orr M, Frieman MB, Baric RS. Severe acute respiratory syndrome coronavirus evades antiviral signaling: role of nsp1 and rational design of an attenuated strain. J Virol. 2007 Nov [PMID: 17715225]
Gupta V, Tabiin TM, Sun K et al. SARS coronavirus nucleocapsid immunodominant T-cell epitope cluster is common to both exogenous recombinant and endogenous DNA-encoded immunogens. Virology. 2006 Mar 30 [PMID: 16387339] (WB)

Confocal, EM
Tseng CT, Tseng J, Perrone L et al. Apical entry and release of severe acute respiratory syndrome-associated coronavirus in polarized Calu-3 lung epithelial cells. J Virol. 2005 Aug [PMID: 16014910] (ICC/IF) ICC/IF
Cate D, Hsieh H, Glukhova V et al. Antibody Screening Results for Anti-Nucleocapsid Antibodies Towards the Development of a SARS-CoV-2 Nucleocapsid Protein Antigen Detecting Lateral Flow Assay ChemRxiv Jul 27 2020
Show All 11 Publications.

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Product General Protocols

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WB Video Protocol
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Gene Symbol N