SARS-CoV-2 Spike Antibody (CR3022) - Chimeric

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ELISA: SARS-CoV-2 Spike Antibody (CR3022) [NBP2-90979] - Binding curve of SARS-CoV-2 Spike Antibody (CR3022) to SARS-CoV-2 Spike Glycoprotein (S1), Sheep Fc-Tag and SARS-CoV-2 Spike Glycoprotein (S2), Sheep Fc-Tag from ...read more
ELISA: SARS-CoV-2 Spike Antibody (CR3022) [NBP2-90979] - Binding curve of SARS-CoV-2 Spike Antibody (CR3022) to SARS-CoV-2 Spike Glycoprotein domains S1 and S2 of various origin. ELISA plate coated with SARS-CoV-2 Spike ...read more

Product Details

Summary
Reactivity VSpecies Glossary
Applications ELISA, SPR
Clone
CR3022
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

SARS-CoV-2 Spike Antibody (CR3022) - Chimeric Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
The original monoclonal antibody was generated by sequencing peripheral blood lymphocytes of a patient exposed to the SARS-CoV.
Specificity
This antibody binds to both SARS-CoV and SARS-CoV-2 with high affinity (PMID: 16796401 & 32065055). It binds the amino acids 318-510 in the S1 domain of the SARS-CoV Spike protein as well as SARS-CoV-2 (COVID-19) Spike protein. The antibody also binds to P462L-substituted S318-510 fragments of the SARS spike protein. The binding epitope is only accessible in the "open" confromation of the spike protein (Joyce et al. 2020).
Isotype
IgG Kappa
Clonality
Monoclonal
Host
Rabbit
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Surface Plasmon Resonance
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.

The initial characterization of the binding of this antibody was performed by ELISA and indicates potential for the development of diagnostic assays, as both virus-capture assays, or as controls in serological assays measuring immune-responses to virus exposure. Human IgG1, IgG3, IgM and IgA isotypes are available to mimic antibody responses seen in COVID19 (Amanat et al. 2020). Human IgG2 is available to assess its yet unknown role in the response to SARS-CoV-2. The original human IgG1 version of the antibody works synergistically in combination with another non-competing SARS antibody CR3014 and is a potential candidate for passive immune prophylaxis of SARS-CoV infection (Meulen et al., 2006). The original antibody (human IgG1) was also reported to bind the 2019-nCoV RBD (KD of 6.3 nM). This antibody has been attributed a potential to be developed as a therapeutic agent, alone or in combination with other neutralizing antibodies for treatment of 2019-nCoV infections (Tian et al., 2020).

Reactivity Notes

SARS-CoV, SARS-CoV-2

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Proclin 300
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for SARS-CoV-2 Spike Antibody (CR3022) - Chimeric

  • 2019-nCoV S Protein
  • 2019-nCoV Spike
  • COVID-19 Spike
  • Human coronavirus spike glycoprotein
  • SARS-COV-2 S protein
  • SARS-COV-2 Spike glycoprotein
  • SARSCOV2 Spike protein
  • Severe Acute Respiratory Syndrome Coronavirus 2 Spike Protein
  • Spike glycoprotein
  • Spike

Background

Coronaviruses are positive-stranded RNA viruses, featuring the largest viral RNA genomes known to date (27-31 kb). Seven coronaviruses had been found to cause human infection including SARS-CoV-2/2019-nCoV resulting in a potentially fatal atypical pneumonia, named COVID-19. SARS-CoV-2 has 4 major structural proteins including spike (S), envelope (E), membrane (M), and nucleocapsid (N), which share significant sequence homology with SARS-CoV. The spike protein in SARS-CoV-2 (1,273 aa) is a viral surface glycoprotein. Research on SARS-host protein interactions with SARS-CoV and SARS-CoV-2 viruses have shown that the viral spike (S) protein binds human ACE-2 located on the surface of mucosal cells, resulting in fusion of viral and cell membranes for viral entry. Viral entry also requires priming of the S protein by host cell proteases such as TMPRSS2, Cathepsin L or furin. The Coronavirus Spike precursor protein is processed into 2 distinct subunits with S1 (654 aa) housing the receptor binding domain (RBD) and S2 (588 aa or 458 a) facilitating membrane fusion.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-90979AF350
Alexa Fluor 405 NBP2-90979AF405
Alexa Fluor 488 NBP2-90979AF488
Alexa Fluor 532 NBP2-90979AF532
Alexa Fluor 594 NBP2-90979AF594
Alexa Fluor 647 NBP2-90979AF647
Alexa Fluor 700 NBP2-90979AF700
Alexa Fluor 750 NBP2-90979AF750
Biotin NBP2-90979B
DyLight 350 NBP2-90979UV
DyLight 405 NBP2-90979V
DyLight 488 NBP2-90979G
DyLight 550 NBP2-90979R
DyLight 594 NBP2-90979DL594
DyLight 650 NBP2-90979C
DyLight 680 NBP2-90979FR
DyLight 755 NBP2-90979IR
FITC NBP2-90979F
HRP NBP2-90979H

Additional SARS-CoV-2 Spike Products

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