RPRD2 Antibody

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Western Blot: RPRD2 Antibody [NB100-366] - Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-KIAA0460 antibody used for WB at 0.2 ug/ml. ...read more
Western Blot: RPRD2 Antibody [NB100-366] - Whole cell lysate (WCL; amounts indicated) from HeLa cells. Antibody used at 1 ug/ml.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
1.0 mg/ml
   

Biological Strategies

     

Order Details

RPRD2 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 700 and 750 of human KIAA0460 using the numbering given in entry CAH70314.1 (GeneID 23248).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
RPRD2
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
Application Notes
By Western blot, this antibody recognizes a band at ~220 kDa, representing hypothetical protein KIAA0460. Not suitable for Immunoprecipitation, use NB100-367 for IP. *The investigator should determine the optimal working dilution for a specific application.
Positive Control
HeLa Lysate (NB800-PC1)

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for RPRD2 Antibody

  • HSPC099
  • KIAA0460FLJ32145
  • regulation of nuclear pre-mRNA domain containing 2
  • regulation of nuclear pre-mRNA domain-containing protein 2

Background

KIAA0460 was first identified as a hypothetical protein predicted from the in silico analysis of long cDNAs isolated in the Kazusa cDNA sequencing project. The function of KIAA0460 has not been characterized. Alternate names for KIAA0082 include HSPC099, and FLJ32145.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol RPRD2
Entrez
Uniprot