RNF220 Overexpression Lysate (Native)

Images

 
Western Blot: RNF220 Overexpression Lysate (Adult Normal) [NBL1-15446] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for RNF220.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

RNF220 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC206907 and based on accession number NM_018150. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens ring finger protein 220 (RNF220), mRNA.
Gene
RNF220

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
62.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for RNF220

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for RNF220 Overexpression Lysate (Native)

  • C1orf164
  • chromosome 1 open reading frame 164
  • E3 ubiquitin-protein ligase RNF220
  • EC 6.3.2.-
  • ring finger protein 220FLJ10597

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-89522
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-03993
Species: Hu, Bv, Ca, Pm
Applications: WB, IHC, IHC-P
NBP2-21037
Species: Hu
Applications: WB, IHC, IHC-P
NBP1-59068
Species: Hu
Applications: WB
NBP2-20367
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P, IP
NBP1-81988
Species: Hu
Applications: IHC, IHC-P

Publications for RNF220 Lysate (NBL1-15446) (0)

There are no publications for RNF220 Lysate (NBL1-15446).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for RNF220 Lysate (NBL1-15446) (0)

There are no reviews for RNF220 Lysate (NBL1-15446). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for RNF220 Lysate (NBL1-15446) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional RNF220 Products

Bioinformatics Tool for RNF220 Lysate (NBL1-15446)

Discover related pathways, diseases and genes to RNF220 Lysate (NBL1-15446). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

PTMs for RNF220 Lysate (NBL1-15446)

Learn more about PTMs related to RNF220 Lysate (NBL1-15446).

Blogs on RNF220

There are no specific blogs for RNF220, but you can read our latest blog posts.

Customers Who Bought This Also Bought

HDAC9 Antibody
NBP2-03993
RNF220 Antibody
NBP2-58631
SIN3B Antibody
NBP2-20367

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our RNF220 Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol RNF220