RNF145 Antibody - BSA Free

Images

 
Immunocytochemistry/ Immunofluorescence: RNF145 Antibody [NBP1-82012] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm, plasma membrane & cytosol.
Immunohistochemistry-Paraffin: RNF145 Antibody [NBP1-82012] - Staining of human skin shows strong cytoplasmic positivity in epidermal cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free

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Catalog# & Formulation Size Price

RNF145 Antibody - BSA Free Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: NSSQLPGLGTEPVLQPHAGAEQNVMFQEGTEPPGQEHTPGTRIQEGSRDNNEYIARRPDNQEGAFDPKEYPHSAKDEAHPVESA
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RNF145
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
RNF145 Protein (NBP1-82012PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for RNF145 Antibody - BSA Free

  • DKFZp686M11215
  • FLJ31951
  • FLJ44310
  • ring finger protein 145

Background

The RNF145 gene encodes a RING finger protein 145 that exists in a total of five isoforms. Isoform 1 is 663 amino acids long and 75 kDA, isoform 2 is 691 amino acid long at 78 kDA, isoform 3 is 680 amino acids long at 77 kDA, isoform 4 is 677 amino acids long at 77 kDA, and isoform 5 is 693 amino acids long at 79 kDA. RNF145 is known to interact with the UBC gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol RNF145