Recombinant Mouse TREML4/TLT-4 Fc Chimera Protein, CF

Recombinant Mouse TREML4/TLT-4 Fc Chimera (Catalog # 10172-TL) binds to apoptotic EL-4 mouse lymphoblasts in a flow cytometry assay. The ED50 for this effect is 0.45‑4.5 μg/mL.

2 μg/lane of Recombinant Mouse TREML4/TLT-4 Fc Chimera (Catalog # 10172-TL)was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditionsand visualized by Coomassie® Bluestaining, showing bands at 64-72 kDa and 120-150 kDa, respectively.

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse TREML4/TLT-4 Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its ability to bind apoptotic EL-4 mouse lymphoblasts in a flow cytometry assay. The ED₅₀ for this effect is 0.45-4.5 μg/mL.
• Source: Mouse myeloma cell line, NS0-derived mouse TREML4/TLT-4 protein
  

  Mouse TREML4/TLT-4 (Ser29-Leu197) Accession # Q3LRV9	IEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #: Q3LRV9
• N-terminal Sequence: Ser29
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 45 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 64-72 kDa, under reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 3 months, -20 to -70 °C under sterile conditions after opening.
• Buffer: Supplied as a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse TREML4/TLT-4 Fc Chimera Protein, CF

• TLT4
• TREML4

Background

TREML4 (Triggering Receptor Expressed on Myeloid cells-like 4), also known as TLT-4, is a type I transmembrane member of the TREM family and Ig superfamily (1, 2). Mature mouse TREML4 consists of a 172 amino acid (aa) extracellular domain (ECD) with one Ig-like domain, a 21 aa transmembrane segment, and a 42 aa cytoplasmic domain (1, 2). Within the ECD, mouse TREML4 shares 51% and 76% aa sequence identity with human and rat TREM-2, respectively. TREML4 is expressed in the spleen, CD8 alpha ⁺ dendritic cells, and macrophages, and it is capable of recognizing necrotic cells by different phagocytes within the spleen (1). TREML4 is characterized by its Ig-like extracellular domain and short cytoplasmic tail that associates with adaptor DAP12 (2). TREML4 has been shown to play a role in coronary calcification and artery disease (3), and it is also a positive regulator of TLR7 signaling in autoimmunity and antiviral responses (4).

1. Hemmi, H. et al. (2009) J. Immunol. 182:1278.
2. Hemmi, H. et al. (2012) J. Immunol. 188:1147.
3. Sen, S.K. et al. (2014) Am. J. Hum. Genet. 95:66.
4. Ramirez-Ortiz, Z. G. et al. (2015) Nat. Immunol. 16:495.

Bioinformatics

• Uniprot:
  • Q3LRV9()