Recombinant Mouse Follistatin-like 5/FSTL5 Protein, CF

Recombinant Mouse Follistatin-like 5/FSTL5 (Catalog #9065-FN) inhibits Hep3B human hepatocellular carcinoma cell survival. The ED50 for this effect is typicallly2-10 μg/mL.

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse Follistatin-like 5/FSTL5 Protein, CF Summary

• Details of Functionality: Measured by its ability to inhibit Hep3B human hepatocellular carcinoma cell survival. The ED₅₀ for this effect is 2-10 μg/mL.
• Source: Chinese Hamster Ovary cell line, CHO-derived mouse Follistatin-like 5/FSTL5 protein
  Arg21-Ala847, with a C-terminal 6-His tag
• Accession #: Q8BFR2
• N-terminal Sequence: Arg21
• Protein/Peptide Type: Recombinant Proteins
• Gene: Fstl5
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 94 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 85-98 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Follistatin-like 5/FSTL5 Protein, CF

• Follistatin like 5
• Follistatin-like 5
• Follistatin-like protein 5
• follistatin-related protein 5
• FSL5
• FSTL5
• KIAA1263DKFZp566D234

Background

Follistatin-like 5 (FSTL5) is a secreted 94 kDa (predicted) member of the SPARC protein family (1). In its N-terminal half, FSTL5 contains a Kazal-like domain, two EF-hand domains, and two Ig-like domains. Mature mouse FSTL5 shares 91% and 97% amino acid sequence identity with human and rat FSTL5, respectively. FSTL5 is expressed in the cartilage and tendon of developing digits and the shafts of long bones (2). In the brain it is expressed in the olfactory bulb, CA3 area of the hippocampus, and granule cell layer of the cerebellum (3). FSTL5 inhibits the proliferation and promotes the apoptosis of hepatocellular carcinoma cells (4). Its expression in medulloblastoma and downregulation in hepatocellular carcinoma are associated with poor prognosis (4, 5).

1. Bradshaw, A.D. (2012) Int. J. Biochem. Cell Biol. 44:480.
2. Lorda-Diez, C.I. et al. (2013) PLoS One 8:e60423.
3. Masuda, T. et al. (2014) Congenit. Anom. (Kyoto) 54:63.
4. Zhang, D. et al. (2015) Int. J. Clin. Exp. Pathol. 8:3386.
5. Remke, M. et al. (2011) J. Clin. Oncol. 29:3852.

Bioinformatics

• Gene Symbol: Fstl5
• Uniprot:
  • Q8BFR2()