Recombinant Mouse Ameloblastin Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse Ameloblastin Protein, CF Summary

• Details of Functionality: Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells. The ED₅₀ for this effect is 1-4 μg/mL.
• Source: Mouse myeloma cell line, NS0-derived mouse Ameloblastin protein
  Val27-Pro422, with a C-terminal 6-His tag
• Accession #: AAH87927
• N-terminal Sequence: Val27 & Ala29
• Protein/Peptide Type: Recombinant Proteins
• Gene: Ambn
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 43 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 70-80 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 250 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Ameloblastin Protein, CF

• AI1F
• AMBN
• Ameloblastin

Background

Ameloblastin (AMBN) is a secreted bone and tooth-specific glycoprotein member of the enamel matrix protein family. Mouse Ameloblastin is synthesized by ameloblasts and osteoblasts as a 407 amino acid (aa; short or “a” isoform) or 422 aa (long or “b” isoform) protein with predicted molecular masses of 44 kDa or 45 kDa, respectively (1). The long isoform includes an additional 15 aa insert following aa 103 and contains additional glycosylation sites (2). This recombinant protein corresponds to the mature long isoform of mouse Ameloblastin. Ameloblastin may be cleaved following secretion leading to the generation of multiple sized proteins from 17-66 kDa (3-6). Mouse Ameloblastin (long isoform) shares 60% and 96% aa sequence identity with the human and rat orthologs, respectively. Ameloblastin is a critical component of tooth enamel and bone matrix where it binds calcium, thereby facilitating bone and tooth mineralization. It has also been shown to self-associate into ribbon-like supramolecular structures (7). During tooth development, it acts by binding to and maintaining the differentiated phenotype of secretory ameloblasts (8, 9). During bone development, Ameloblastin regulates osteogenic differentiation by binding to CD63 and promoting CD63-Integrin beta 1 interactions (10). Ameloblastin may also promote osteoclastogenesis (11). Ameloblastin can act as an adhesion molecule and promotes the adhesion of periodontal ligament cells via RhoA activation and ameloblasts via heparin-binding domains (8, 12, 13).

1. Simmons, D. et al. (1998) Connect Tissue Res. 39:3.
2. Kobayashi, K. et al. (2007) J. Dent. Res. 86:962.
3. Uchida, T. et al. (1997) J. Histochem. Cytochem. 45:1329.
4. Chun, Y.H. et al. (2010) J. Dent. Res. 89:785.
5. Ravindranath, R.M. et al. (2007) J. Biol. Chem. 282:36370.
6. Yamakoshi, Y. et al. (2011) Eur. J. Oral Sci. 119 Suppl 1:206.
7. Wald, T. et al. (2013) J. Biol. Chem. 288:22333.
8. Fukumoto, S. et al. (2004) J. Cell Biol. 167:973.
9. Fukumoto, S. et al. (2005) Cells Tissues Organs 181:189.
10. Iizuka, S. et al. (2011) Mol. Cell Biol. 31:783.
11. Lu, X. et al. (2013) Bone 54:157.
12. Zhang, Y. et al. (2011) Eur. J. Oral. Sci. 119:Suppl 1:280.
13. Sonoda, A. et al. (2009) J. Biol. Chem. 284:27176.

Bioinformatics

• Gene Symbol: Ambn
• Uniprot:
  • AAH87927()