Recombinant Human MFAP3 Fc Chimera Protein, CF

2 μg/lane of Recombinant Human MFAP3 Fc Chimera (Catalog # 10800-MF) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 57-67 kDa and 114-134 kDa, respectively.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human MFAP3 Fc Chimera Protein, CF Summary

• Details of Functionality: Bioassay data are not available.
• Source: Human embryonic kidney cell, HEK293-derived human MFAP3 protein
  

  Human MFAP3 (Leu22-Met147) Accession # P55082.1	IEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #: P555082.1
• N-terminal Sequence: Leu22
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 41 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 57-67 kDa, under reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 µg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human MFAP3 Fc Chimera Protein, CF

• DKFZp586F0219
• MFAP3
• microfibril-associated glycoprotein 3
• microfibrillar-associated protein 3

Background

Microfibril-associated glycoprotein 3 (MFAP3), is a member of the MFAP family which play a role in microfibril assembly, elastinogenesis, and tissue homeostasis. The family members were identified as potential components of fibrillin-containing microfibrils, but they do not share structural similarities (1). MFAP3 is found in ocular zonules. Human MFAP3 is a serine-rich acidic protein located in zonular fibers and consists of an extracellular domain (ECD) containing one Ig-like C2-type domain, a single transmembrane domain, and a cytoplasmic domain. Within the ECD, mature human MFAP3 shares 74% amino acid sequence identity with mouse and rat MFAP3. Among MFAP3-related pathways are degradation of the extracellular matrix and elastic fiber formation. MFAP is a candidate gene for heritable diseases affecting microfibrils (2).

1. Gibson, M.A. Landes Bioscience https://www.ncbi.nlm.nih.gov/books/NBK6448/.
2. Abrams W.R. (1995) Genomics 26:47.

Bioinformatics

• Uniprot:
  • P555082.1()