Recombinant Human Ly6H His-tag Protein, CF

2 μg/lane of Recombinant Human Ly6H His-tag Protein (Catalog # 10373-L6) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 13-25 kDa.

Measured by its ability to inhibit anti-CD3 antibody induced IL-2 secretion by human T cells. The ED50 for this effect is 0.75-7.5 μg/mL.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human Ly6H His-tag Protein, CF Summary

• Details of Functionality: Measured by its ability to inhibit anti-CD3 antibody induced IL-2 or IFN-gamma secretion by human T cells. The ED₅₀ for this effect is 0.75-7.5 μg/mL
• Source: Chinese Hamster Ovary cell line, CHO-derived human Ly6H protein
  Leu26-Gly115, with a C-terminal 6-His tag
• Accession #: O94772.1
• N-terminal Sequence: Leu26
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 10.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 13-25 kDa, under reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 200 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Ly6H His-tag Protein, CF

• Ly6H
• Ly-6H
• NMLy6

Background

Lymphocyte Antigen 6 Family Member H (Ly6H), also known as NMLy6, is a member of the Ly6/urokinase-type plasminogen activator receptor (uPAR) superfamily of proteins. LY6 proteins are primarily glycosylphosphatidylinositol (GPI)-anchored cell surface glycoproteins characterized by the presence of an LU domain. The LU domain is an ~80 amino acid (aa) region with a conserved disulfide bridge arrangement resulting in a three-fingered structural motif (1). The mature extracellular domain of human Ly6H shares 92% aa sequence identity with mouse Ly6H. Ly6H is predominantly expressed in normal brain tissue and can modulate alpha 7 nicotinic acetylcholine receptor ( alpha 7 nAChR) signaling (2, 3). The alpha 7 nAChR signaling is associated with several conditions, such as nicotine addiction, lung and liver cancer, preeclampsia, schizophrenia, and Alzheimer's disease (2, 3). The alpha 7-subunits are controlled by prototoxins in the Ly6 family. Specifically, Ly6H reduces cell-surface expression and calcium signaling by alpha 7 nAChRs, thus suppressing alpha 7 nAChR function (2, 3). Ly6H expression is increased in tumors associated with several cancer types (4). The increase in expression is associated with poor outcome on patient survival in breast, lung, ovarian, gastric, and colorectal cancer (4).

1. Upadhyay, G. et al. (2019) Front Immunol. 10:819.
2. Puddifoot, C. A. et al. (2015) J Neurosci. 35:3420.
3. Govind, A. et al. (2012) J Neurosci. 32:2227.
4. Luo, L. et al. (2016) Oncotarget. 7:11165.

Bioinformatics

• Uniprot:
  • O94772.1()