Recombinant Human LRRTM4 Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human LRRTM4 Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When rhLRRTM4 is coated at 5 μg/mL (100 μL/well), rhNeurexin 1 beta /Fc Chimera (Catalog # 5268-NX) binds with an apparent K_D <15 nM.
• Source: Mouse myeloma cell line, NS0-derived human LRRTM4 protein
  Gln31-Lys424, with a C-terminal 6-His tag
• Accession #: Q86VH4
• N-terminal Sequence: No results obtained: Gln31 predicted
• Protein/Peptide Type: Recombinant Proteins
• Gene: LRRTM4
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 46.0 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 60-70 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 300 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human LRRTM4 Protein, CF

• FLJ12568
• leucine rich repeat transmembrane neuronal 4
• leucine-rich repeat transmembrane neuronal 4 protein
• leucine-rich repeat transmembrane neuronal protein 4
• LRRTM4
• MGC120633
• MGC120636

Background

LRRTM4 (Leucine-rich repeat transmembrane protein 4) is a member of the LRRTM family of molecules (1). All LRRTMs are type I transmembrane proteins that contain multiple leucine rich repeats, one PDZ consensus cytoplasmic binding domain, and show expression somewhere in the central nervous system. The LRRTM family is iterated across vertebrate (but not invertebrate) species. Human LRRTM4 is synthesized as a 590 amino acid (aa) precursor that contains a 30 aa signal sequence plus a 560 aa mature region. The mature molecule contains a 394 aa extracellular domain (aa 31 ‑ 424), a 21 aa transmembrane segment, and a 105 aa cytoplasmic region (1, 2). The extracellular domain is characterized by the presence of ten Leu-rich repeats, flanked by two cysteine-rich flanking sequences. Based on other LRRTMs, eukaryotic expression of LRRTM4 will likely yield an 82 ‑ 86 kDa glycosylated molecule (3, 4). There are at least two splice variants. Both show a Val substitution for aa 518 ‑ 590, one of which is also coupled to an alternative start site at Met312 (5, 6). Mature human LRRTM4 is 95% aa identical to mouse LRRTM4 (1). LRRTM4 is widely expressed, occurring in embryonic distal limb bud mesenchyme plus anterior sclerotome, and in adult testis, lung, muscle and prostate (1, 7). All LRRTMs are also found in brain. LRRTM4 has been identified in adult granular and mitral neurons of the olfactory bulb, neurons of cerebral cortex layers 2, 4, 5 and 6, and on neurons of the dentate gyrus (1). Functionally, it appears that LRRTM4 is involved in both pre-and post-synaptic modeling. Although embedded in the postsynaptic membrane, LRRTM4 seems to induce excitatory/glutamatergic presynaptic differentiation. Postsynaptically, LRRTM4 likely facilitates excitatory synapse formation on dendrites, acting in conjunction with multiple ligand-receptor combinations. In particular, the NR1 NMDA subunit has been shown to coalesce, or aggregate, in regions that experience LRRTM4 clustering (3, 8).

1. Lauren, J. et al. (2003) Genomics 81:411.
2. SwissProt # Q86VH4.
3. Linhoff, M.W. et al. (2009) Neuron 61:734.
4. Majercak, J. et al. (2006) Proc. Natl. Acad. Sci. USA 103:17967.
5. GenBank Accession # EAW99585.
6. GenBank Accession # AAH17769.
7. Haines, B.P. & P. Rigby (2007) Gene Expr. Patterns 7:23.
8. Brose, N. (2009) Neuron 61:650.

Bioinformatics

• Gene Symbol: LRRTM4
• Uniprot:
  • Q86VH4()