Recombinant Human ILDR2 Fc Chimera Protein, CF

Recombinant Human ILDR2 Fc Chimera (Catalog # 9991-IL) inhibits IFNgsecretion by human peripheral blood mononuclear cells in the presence ofanti-CD3 antibody. The ED50 for this effect is 1‑6 μg/mL.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human ILDR2 Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its ability to inhibit anti-CD3 antibody induced IFN-gamma secretion by human peripheral blood mononuclear cells (PBMC). The ED₅₀ for this effect is 1-6 μg/mL.
• Source: Chinese Hamster Ovary cell line, CHO-derived human ILDR2 protein
  

  Human ILDR2 (Leu21 - Glu186) Accession # Q71H61	IEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #: Q71H61
• N-terminal Sequence: Leu21
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 45 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 47-52 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage:
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 200 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human ILDR2 Fc Chimera Protein, CF

• Angulin-3
• C1orf32
• Dbsm1
• DJ782G3.1
• ILDR2
• immunoglobulin-like domain containing receptor 2
• LISCH-Like

Background

ILDR2 (Immunoglobulin-like domain-containing receptor 2) is a member of the B7-like family of proteins that regulate T cell activity (1). ILDR2 is also a known endoplasmic reticulum molecule that regulates lipid homeostasis (2, 3). It contains a signal peptide, an Ig-like V-type domain, a stalk region, a transmembrane domain and a CCP-rich domain (1, 4). The human ILDR2 lumenal domain shares a 99% and 98% homology with the mouse and rat respectively. The human gene encoding ILDR2 is located in a region on Chr1q23–25 that has been associated with type 2 diabetes (5). ILDR2 and its two paralogs, ILDR1 and lipolysis-stimulated receptor (LSR; also named ILDR3), are members of angulin family proteins (angulin-1/LSR, angulin-2/ILDR1, and angulin-3/ILDR2), and they are identified as protein components of tricellular tight junctions (tTJs), which are required for recruitment of tricellulin to tTJs (6). ILDR2 plays critical roles in hepatic clearance of lipoproteins and in lipid homeostasis (3). ILDR2 regulates human dendritic cells (DC2 cells, a subpopulation of polarized DCs that promotes Th2 differentiation) (7). Recent publications reported that ILDR2 displayed negative regulatory functions on human and mouse T cells in various experimental systems. Fusion protein of ILDR2 lumenal domain with an Fc fragment, displays therapeutic effects in collagen-induced arthritis (CIA), a mouse model of rheumatoid arthritis (RA). ILDR2 represents a novel B7-like ligand that exerts negative immune modulation via interaction with a putative counterpart receptor expressed on activated T cells (1, 4).

1. Hecht, I. et al. (2018) J. Immunol. 200:2025.
2. Watanabe, K. et al. (2013) PLoS One. 8:e67234.
3. Watanabe, K. et al. (2016) Biochem. Biophys. Res. Commun. 477:712.
4. Podojil, J. R. et al. (2018) J. Immunol. 200:2013.
5. Dokmanovic-Chouinard, M. et al. (2008) PLoS Genet. 4:e1000137.
6. Higashi, T. et al. (2013) J. Cell Sci. 126:966.
7. Gueguen, C. et al. (2016) J. Allergy Clin. Immunol. 137:545.

Bioinformatics

• Uniprot:
  • Q71H61()