Recombinant Human EMR2 Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Human EMR2 Protein, CF Summary

Details of Functionality
Measured by the ability of the immobilized protein to support the adhesion of NIH‑3T3 mouse embryonic fibroblast cells. Stacey, M. et al. (2003) Blood 102:2916. rhEMR2 immobilized at 2 µg/mL, 100 µL/well, will mediate >45% NIH/3T3 cell adhesion (1 x 105 cells/well).
Source
Chinese Hamster Ovary cell line, CHO-derived human EMR2 protein
Gln24-Gln478, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
No results obtained: Gln24 predicted
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
ADGRE2
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
50.5 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
80-105 kDa, reducing conditions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human EMR2 Protein, CF

  • CD312
  • DKFZp781B135
  • egf-like module containing, mucin-like, hormone receptor-like 2
  • egf-like module containing, mucin-like, hormone receptor-like sequence 2
  • EGF-like module receptor 2
  • EGF-like module-containing mucin-like hormone receptor-like 2
  • EMR2

Background

EMR2 (EGF-like module-containing mucin-like receptor 2; designated CD312) is a glycoprotein belonging to the EGF-TM7 family of adhesion-type class B 7-transmembrane (TM) receptors. EGF-like sequences within long extracellular N-termini, and a GPS (G-protein proteolytic site) domain are characteristic of this family, which is mainly expressed on cells of the immune system (1, 2). The human EMR2 cDNA encodes an 823 amino acid (aa) protein with five EGF-like domains within the first 250 aa, followed by a mucin-like stalk, a GPS domain (aa 479 - 530) and a 7-TM sequence (aa 531 - 785). The GPS domain is the site of autocatalytic cleavage, forming two cleaved portions that remain non-covalently attached as a heterodimer (1, 3). Of the first 290 aa of human EMR2, 284 aa (97%) are identical with family member CD97, likely due to gene duplication (2). The portion of human EMR2 N-terminal to the GPS domain (aa 1 - 478) shares 64%, 59%, 48% and 45% aa identity with corresponding regions of canine EMR2, equine EMR2, mouse CD97 and rat CD97, respectively. Alternate splicing of EMR2 creates isoforms that contain 2 - 5 EGF-like domains. Only the 5-EGF form contains EGF4, which is necessary for calcium-dependent binding of the EMR2/CD97 ligand, chondroitin sulfate (CS) (2, 4 - 6). None of the isoforms engage the CD97 ligand, CD55 (DAF). EMR2 is restricted to myeloid cells (1, 2). EMR2 expression increases as monocytes differentiate into macrophages, and decreases with differentiation into dendritic cells (5). Activation increases neutrophil EMR2 expression (5). EMR2 localizes to the leading edge of migrating neutrophils and plays an important role in migration, adhesion and superoxide production (7). It is also thought to facilitate specific interaction of myeloid cells with peripheral B lymphocytes which express CS (6).

  1. Kwakkenbos, M.J. et al. (2004) Immunogenetics 55:655.
  2. Lin, H.-H. et al. (2000) Genomics 67:188.
  3. Lin, H.-H. et al. (2004) J. Biol. Chem. 279:31823.
  4. Stacey, M. et al. (2003) Blood 102:2916.
  5. Chang G.-W. et al. (2007) Biochem. Biophys. Res. Commun. 353:133.
  6. Kwakkenbos, M.J. et al. (2005) J. Leukoc. Biol. 77:112.
  7. Yona, S. et al. (2008) FASEB J. 22:741.

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Bioinformatics

Gene Symbol ADGRE2
Entrez
Uniprot