Recombinant Human CEACAM-16 Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human CEACAM-16 Protein, CF Summary

• Details of Functionality: Bioassay data are not available.
• Source: Chinese Hamster Ovary cell line, CHO-derived human CEACAM-16 protein
  Met1-Gly425, with a C-terminal HA-tag
• Accession #: Q2WEN9
• N-terminal Sequence: Glu21
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 45 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 56-73 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 250 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human CEACAM-16 Protein, CF

• carcinoembryonic antigen-related cell adhesion molecule 16
• CEACAM-16
• CEAL2
• DFNA4B

Background

Carcinoembryonic Antigen-related Cell Adhesion Molecule 16 (CEACAM-16), or CEAL2, is part of the CEA protein family consisting of CEACAMs and the pregnancy‑specific glycoproteins (PSGs). Both CEACAM and PSG molecules have been identified in humans and belong to the much larger glycosylphosphatidylinositol (GPI)-linked immunoglobulin (Ig) superfamily (1, 2). Unique to the CEA family, CEACAM-16 is a secreted molecule lacking a recognizable transmembrane domain or GPI anchor (3). Mature human CEACAM-16 is 405 amino acids (aa), containing 2 IgC2-like domains and 2 IgV-like domains. CEACAM-16 is one of only five conserved CEACAMs among mice, rats, and humans (2). Mature human CEACAM-16 shares 90% and 89% aa identity with mouse and rat CEACAM‑16, respectively. Originally discovered as a biomarker for colorectal cancer (4), CEACAMs have now been associated with numerous intracellular signaling processes including cell adhesion, cell growth, recognition and differentiation, angiogenesis, and apoptosis (5-7). CEACAM-16 is specifically expressed in the inner ear and has been shown to play a critical role in hearing. CEACAM-16 has been identified as a binding partner for alpha tectorin and specific mutations in CEACAM-16 have been linked to autosomal dominant nonsyndromic deafness (ADNSHL) (3, 8).

1. Beauchemin, N. et al. (1999) Exp. Cell Res. 252:243.
2. Zebhauser R. et al. (2005) Genomics 86:566.
3. Zheng, J. et al. (2011) PNAS. 108(10):4218.
4. Gold P. and Freedman S.O. (1965) J Exp Med 122:467.
5. Obrink, B. (1997) Curr Opin Cell Biol 9:616.
6. Horst, A.K. and Wagener, C. (2004) Handb Exp Pharmacol 283.
7. Kuespert K et al. (2006) Curr Opin Cell Biol. 18(5):565.
8. Wang, H. et al. (2015) J Hum Genet. 60(3):119.

Bioinformatics

• Uniprot:
  • Q2WEN9()