Recombinant Human AMIGO3 Fc Chimera Protein, CF

Recombinant Human AMIGO3 Fc Chimera (Catalog # 9508‑AM)inhibits IL-2 secretion by mouse T cells in the presence of anti-CD3 antibody.The ED50 for this effect is 1-5 μg/mL.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human AMIGO3 Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its ability to inhibit IL-2 secretion by mouse T cells in the presence of anti-CD3. The ED₅₀ for this effect is 1-5 μg/mL.
• Source: Chinese Hamster Ovary cell line, CHO-derived human AMIGO3 protein
  

  Human AMIGO3 (Thr20-Pro378) Accession # Q86WK7	IEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #: Q86WK7
• N-terminal Sequence: Thr20
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 66 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 83-103 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 200 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human AMIGO3 Fc Chimera Protein, CF

• adhesion molecule with Ig-like domain 3
• ALI3
• Alivin-3
• AMIGO3
• AMIGO-3
• amphoterin-induced gene and ORF 3
• amphoterin-induced protein 3
• KIAA1851
• MGC120552

Background

AMIGO3 (Amphoterin-induced protein 3), also called Alivin-3, is a 68 kDa single-pass type I membrane cell adhesion protein. AMIGO3 belongs to a family of leucine‑rich repeat (LRR) containing proteins that play various roles in nervous system development and function (1, 2). Human AMIGO3 cDNA encodes 504 amino acids (aa) including a 19 aa signal sequence, 264 aa extracelluar domain, 21 aa transmembrane domain and 100 aa cytoplasmic domain. Human AMIGO3 shares 77.2% and 79.1% aa sequence identity with mouse and rat AMIGO3, respectively. AMIGO family proteins are cell adhesion molecules that exhibit homophilic and heterophilic binding properties and are thought to play roles in neuronal axon tract development and cell adhesion. AMIGO3 is expressed in brain tissues, also found in liver, kidney and spleen (3). AMIGO3 mRNA and protein levels are preferentially and significantly raised in DRGN and RGC immediately after central axotomy. Depression of AMIGO3 expression correlates with dorsal column (DC) and optic nerve regeneration. AMIGO3 interacts with NgR1 and p75/TROY forming a functional receptor complex that activates RhoGTP in cells exposed to CNS myelin extracts (CME). AMIGO3 substitutes for LINGO-1 in centrally axotomized DRGN and RGC in the acute phase of injury. AMIGO3-NgR1-p75/TROY receptor complex mediates immediate axon growth inhibitory responses to CNS myelin (4). Our in-house data showed that AMIGO3 inhibited anti-CD3 induced IL-2 secretion on CD3⁺ cells, suggesting that AMIGO3 may be involved in T cell activation.

1. Chen, Y. et al. (2006) Brain Res. Rev. 51:265.
2. SwissProt Accession # Q86WK7.
3. Kuja-Panula J, et al. (2003) J Cell Biol 160:963.
4. Ahmed Z, et al. (2013) PLoS One. 8:e61878.

Bioinformatics

• Uniprot:
  • Q86WK7()