Rattin Antibody - BSA Free

Western Blot: Rattin Antibody [NB300-246] - Analysis of Rattin using NB300-246 using 100 ng of Rattin peptide and from 50 ug of total lysates of 293 T cells transfected with plasmids encoding GFP-Rattin.

HNG decreases Rattin expression in gentamicin-exposed cochlear hair cells.The expression of Rattin in OC explants was measured by real-time PCR. OC explants were treated with 200 μM gentamicin (GM) in combination with or without 1 uM HNG (A) or 0.1 uM SHLP3 (B) for 6 h and 16 h. At least three explants were used as sample pool for each condition. Values are presented as mean+S.D. *P < 0.05, ns no significant between all groups. C Western blot results showing the expression of RATTIN after 24-h peptide treatment (1 uM HNG or 0.08 uM SHLP3). At least six explants were used as a sample pool for each condition. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/39433756), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Rattin Antibody - BSA Free Summary

• Immunogen: A synthetic peptide mapping to residues 20-35 [VKRLESPNKTRRPYGA] of the Rattin protein.
• Specificity: Reacts with residues 20-35 [VKRLESPNKTRRPYGA] of the Rattin peptide, which is not in the conserved region to Humanin.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500
• Application Notes: Use in Immunohistochemistry-Paraffin reported in scientific literature (PMID:32444831).Western blot The investigator should determine the optimal dilution for a specific application such as immunohistochemistry.

Reactivity Notes

This antibody reacts with the rat protein. Use in Human reported in scientific literature (PMID:32444831).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.1% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Rattin Antibody - BSA Free

• Humanin-like in rat
• rattin humanin-like

Background

A novel rat gene, Rattin, encodes a peptide homologous to Humanin, a secreted peptide that specifically protects against neuronal cell death induced by beta-amyloid peptide (Ab) or by mutations causing early-onset familial Alzheimer's disease. Rattin is the prototype of a novel class of peptides, phylogenetically related to Humanin, endowed with protective activity not only against Ab but also toward excitotoxic neuronal death. The rat gene, Rattin, encodes a peptide of 38 residues (15 residues longer than Humanin) showing 73% identity in the conserved region to Humanin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Rattin Antibody (NB300-246)(4)

Publications using NB300-246

• Lu Y, Bartoszek EM, Cortada M et al. Mitochondrial-derived peptides, HNG and SHLP3, protect cochlear hair cells against gentamicin Cell Death Discovery 2024-10-21 [PMID: 39433756]
• Pe�a Agudelo JA, Pidre ML, Garcia Fallit M et al. Mitochondrial Peptide Humanin Facilitates Chemoresistance in Glioblastoma Cells Cancers (Basel) 2023-08-11 [PMID: 37627089] (Western Blot, Rat)
• Moreno Ayala MA, Gottardo MF, Zuccato CF et al. Humanin Promotes Tumor Progression in Experimental Triple Negative Breast Cancer Sci Rep 2020-05-22 [PMID: 32444831] (IHC-P, Human)
• Merdzo I, Rutkai I, Sure VNLR et al. Effects of prolonged type 2 diabetes on mitochondrial function in cerebral blood vessels Am. J. Physiol. Heart Circ. Physiol. 2019-09-06 [PMID: 31490734] (WB, Rat)