PRMT1 Antibody (JA11-17)

Immunocytochemistry/Immunofluorescence: PRMT1 Antibody (JA11-17) [NBP2-67074] - Staining PRMT1 in NIH-3T3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: PRMT1 Antibody (JA11-17) [NBP2-67074] - Analysis of paraffin-embedded mouse liver tissue using anti-PRMT1 antibody. Counter stained with hematoxylin.

Immunocytochemistry/Immunofluorescence: PRMT1 Antibody (JA11-17) [NBP2-67074] - Staining PRMT1 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: PRMT1 Antibody (JA11-17) [NBP2-67074] - Staining PRMT1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: PRMT1 Antibody (JA11-17) [NBP2-67074] - Staining PRMT1 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: PRMT1 Antibody (JA11-17) [NBP2-67074] - Staining PRMT1 in D3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: PRMT1 Antibody (JA11-17) [NBP2-67074] - Analysis of paraffin-embedded human breast cancer tissue using anti-PRMT1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: PRMT1 Antibody (JA11-17) [NBP2-67074] - Analysis of paraffin-embedded mouse colon tissue using anti-PRMT1 antibody. Counter stained with hematoxylin.

Product Discontinued

PRMT1 Antibody (JA11-17) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human PRMT1 aa 12-61 / 371. (SwissProt: Q99873 Human; SwissProt: Q9JIF0 Mouse; SwissProt: Q63009 Rat)
• Localization: Nucleus. Cytoplasm.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PRMT1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for PRMT1 Antibody (JA11-17)

• ANM1
• EC 2.1.1
• EC 2.1.1.-
• EC 2.1.1.125
• EC 2.1.1.77
• HCP1
• Histone-arginine N-methyltransferase PRMT1
• HMT1 (hnRNP methyltransferase, S. cerevisiae)-like 2
• HMT1 hnRNP methyltransferase-like 2 (S. cerevisiae)
• HMT1
• HMT2
• HRMT1L2
• HRMT1L2HMT1 hnRNP methyltransferase-like 2
• Interferon receptor 1-bound protein 4
• IR1B4
• IR1B4protein arginine N-methyltransferase 1
• PRMT1
• protein arginine methyltransferase 1

Background

PRMTs are a family of proteins that either monomethylate or dimethylate the guanidino nitrogen atoms of arginine side chains. This common post-translational modification is implicated in protein trafficking, signal transduction, and transcriptional regulation. Most methyltransferases use the methyl donor, S-adenosyl-L-methionine (AdoMet), as a cofactor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PRMT1