pre-mRNA cleavage factor I (59 kDa subunit) Antibody

Images

 
Western Blot: pre-mRNA cleavage factor I (59 kDa subunit) Antibody [NBP1-89868] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunocytochemistry/ Immunofluorescence: pre-mRNA cleavage factor I (59 kDa subunit) Antibody [NBP1-89868] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.
Immunohistochemistry-Paraffin: pre-mRNA cleavage factor I (59 kDa subunit) Antibody [NBP1-89868] - Staining of human esophagus shows strong nuclear positivity in squamous epithelial cells.
Western Blot: pre-mRNA cleavage factor I (59 kDa subunit) Antibody [NBP1-89868] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

pre-mRNA cleavage factor I (59 kDa subunit) Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids:SDDRSSSTEPPPPVRQEPSPKPNNKTPAILYTYSGLRNRRAAVYVGSFSWWTTDQQLIQVIRSIGVYDVVELKFAENRA
Specificity
Specificity of antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CPSF7
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:500
  • Immunocytochemistry/Immunofluorescence 1 - 4 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:20-1:50
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
pre-mRNA cleavage factor I (59 kDa subunit) Protein (NBP1-89868PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for pre-mRNA cleavage factor I (59 kDa subunit) Antibody

  • CFIm59
  • cleavage and polyadenylation specific factor 7, 59kDa
  • CPSF 59 kDa subunit
  • FLJ12529,59 kDa subunit
  • FLJ39024
  • MGC9315
  • pre-mRNA cleavage factor I, 59 kDa subunit

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional pre-mRNA cleavage factor I (59 kDa subunit) Products

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Bioinformatics

Gene Symbol CPSF7