pre-mRNA cleavage factor I (59 kDa subunit) Antibody

Western Blot: pre-mRNA cleavage factor I (59 kDa subunit) Antibody [NB100-61599] - Detection of Human CPSF59 on HeLa whole cell lysate using NB100-61599. CPSF59 was also immunoprecipitated by rabbit anti-CPSF59 antibody NB100-61600.

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

pre-mRNA cleavage factor I (59 kDa subunit) Antibody Summary

• Description: Novus Biologicals Rabbit pre-mRNA cleavage factor I (59 kDa subunit) Antibody (NB100-61599) is a polyclonal antibody validated for use in WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 1 and 50 of human cleavage and polyadenylation specificity factor 7, 59 kDa subunit using the numbering given in entry NP_079087.2 (GeneID 79869).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CPSF7
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Theoretical MW: 52 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  pre-mRNA cleavage factor I (59 kDa subunit) Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for pre-mRNA cleavage factor I (59 kDa subunit) Antibody

• CFIm59
• cleavage and polyadenylation specific factor 7, 59kDa
• CPSF 59 kDa subunit
• FLJ12529,59 kDa subunit
• FLJ39024
• MGC9315
• pre-mRNA cleavage factor I, 59 kDa subunit

Background

CPSF59 is the the 59 kDa subunit of the cleavage factor Im complex (CFIm). The CFIm is a heterodimer composed of a 25 kDa (CPSF5) subunit and either the 59 kDa (CPSF7) or 68 kDa (CPSF6) subunit and is essential to the first step of pre-mRNA 3'-end processing. CPSF59 bears an RNA-recognition motif (RRM).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CPSF7
• Entrez:
  • 79869(Human)
• Uniprot:
  • NP_079087.2(Human)