PRDM11 Antibody

Western Blot: PRDM11 Antibody [NB100-1525] - (2ug/ml) staining of A549 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: PRDM11 Antibody [NB100-1525] - Staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu
• Applications: WB, IHC, IHC-P, PEP-ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

PRDM11 Antibody Summary

• Immunogen: Peptide with sequence LSEGRVRSGLCGG corresponding to C-Terminus according to NP_064614.2.
• Epitope: C Terminus
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PRDM11
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 2 - 3 ug/ml
• Application Notes: WB: Approx. 50 kDa band observed in human lung carcinoma A549 lysates (calculated MW of 58 kDa band according to NP_064614.2).
  IHC: Paraffin embedded Human Brain (Cortex).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PRDM11 Antibody

• PFM8PR domain-containing protein 11
• PR domain containing 11
• PR-domain containing protein 11

Background

The newly discovered family of PR domain genes is involved in tumorigenesis. Similar to acetylation and phosphorylation, histone methylation at the N-terminal tail has emerged as an important role in regulating chromatin dynamics and gene activity. Histone methylation occurs on arginine and lysine residues and is catalyzed by two families of proteins, the protein arginine methyltransferase family and the SET-domain containing methyltransferase family. Five members have been identified in the arginine methyltransferase family. About 27 are grouped into the SET-domain family, and another 17 make up the PR domain family that is related to the SET domain family. The retinoblastoma protein interacting zinc finger gene RIZ1 is a tumor suppressor gene and a FOUNDING member of the PR domain family. RIZ1 inactivation is commonly found in many types of human cancers and occurs through loss of mRNA expression, frame shift mutation, chromosomal deletion, and missense mutation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PRDM11 Antibody (NB100-1525)(1)

Publication using NB100-1525

• Xiao,B, Wilson,JR Gamblin,SJ. SET domains histone methylation Curr. Opin Struct Biol 13 (6), 699-705. 2003-01-01 [PMID: 14675547]

Bioinformatics

• Gene Symbol: PRDM11
• Entrez:
  • 56981(Human)