Polypeptide GalNac Transferase 10/GALNT10 Antibody [Janelia Fluor® 646]

• Reactivity: Hu, Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Janelia Fluor 646

Polypeptide GalNac Transferase 10/GALNT10 Antibody [Janelia Fluor® 646] Summary

• Description: This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
  
  For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Polypeptide GalNac Transferase 10/GALNT10
  Leu71-Asn603
  Accession # Q86SR1
• Specificity: Detects human and mouse Polypeptide GalNac Transferase 10/GALNT10 in Western blots. In direct ELISA, less than 1% cross-reactivity with recombinant human (rh) GALNT1 and rhGALNT4 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: GALNT10
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot
• Application Notes: Recommended applications are based on validated applications from the unconjugated base product AF7575. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Antigen Affinity-purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for Polypeptide GalNac Transferase 10/GALNT10 Antibody [Janelia Fluor® 646]

• DKFZp586H0623
• EC 2.4.1.41
• FLJ00205
• FLJ11715
• GalNAc transferase 10
• GALNACT10
• GalNAc-T10
• GALNT10
• Polypeptide GalNAc transferase 10
• polypeptide N-acetylgalactosaminyltransferase 10
• PPGALNACT10
• pp-GaNTase 10
• PPGANTASE10
• Protein-UDP acetylgalactosaminyltransferase 10
• UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 10
• UDP-N-acetyl-alpha-D-galactosamine:polypeptideN-acetylgalactosaminyltransferase 10 (GalNAc-T10)

Background

The GALNT10 gene codes for a member of the GalNAc polypeptide N-acetylgalactosaminyltransferases. The enzyme catalyzes the initial reaction in O-linked (mucin-type) oligosaccharide biosynthesis. It creates four isoforms: isoform 1: 603 AA long, nearly 69 kDA; isoform 2: 541 AA long; nearly 62 kDA; isoform 3: 366 AA long; 41 kDA; and isoform 4: 276 AA long: approximately 31 kDA. The GALNT10 gene has been studied with its contribution to thyroiditis. It interacts with genes MUC7, MUC1, GALNT1, GALNT12, and PTCH1 in the metabolism of proteins, mucin type O-glycan biosynthesis, and in post-translational protein modification.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GALNT10