Polypeptide GalNac Transferase 10/GALNT10 Antibody [CoraFluor™ 1]

CoraFluor™ 1, amine reactive (Catalog:7920) and CoraFluor™ 2, amine reactive (Catalog # 7950) are terbium-based probes that have been developed for use as TR-FRET donors. They emit wavelengths compatible with commonly used fluorescent acceptor dyes such as BODIPY® (or BDY) and Janelia Fluor® dyes, FITC (Catalog # 5440), TMR and Cyanine 5 (Catalog # 5436). CoraFluor™ fluorescence is brighter and more stable in biological media than existing TR-FRET donors, leading to enhanced sensitivity and improved data generation. CoraFluor™ 1 exhibits excitation upon exposure to a 337 nm UV laser.

• Reactivity: Hu, Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: CoraFluor 1

Polypeptide GalNac Transferase 10/GALNT10 Antibody [CoraFluor™ 1] Summary

• Description: CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(TM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
  
  CoraFluor(TM) 1, amine reactive
  
  CoraFluor(TM) 1, thiol reactive
  
  For more information, please see our CoraFluor(TM) TR-FRET technology flyer.
• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Polypeptide GalNac Transferase 10/GALNT10
  Leu71-Asn603
  Accession # Q86SR1
• Specificity: Detects human and mouse Polypeptide GalNac Transferase 10/GALNT10 in Western blots. In direct ELISA, less than 1% cross-reactivity with recombinant human (rh) GALNT1 and rhGALNT4 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: GALNT10
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark. Do not freeze.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Antigen Affinity-purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for Polypeptide GalNac Transferase 10/GALNT10 Antibody [CoraFluor™ 1]

• DKFZp586H0623
• EC 2.4.1.41
• FLJ00205
• FLJ11715
• GalNAc transferase 10
• GALNACT10
• GalNAc-T10
• GALNT10
• Polypeptide GalNAc transferase 10
• polypeptide N-acetylgalactosaminyltransferase 10
• PPGALNACT10
• pp-GaNTase 10
• PPGANTASE10
• Protein-UDP acetylgalactosaminyltransferase 10
• UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 10
• UDP-N-acetyl-alpha-D-galactosamine:polypeptideN-acetylgalactosaminyltransferase 10 (GalNAc-T10)

Background

The GALNT10 gene codes for a member of the GalNAc polypeptide N-acetylgalactosaminyltransferases. The enzyme catalyzes the initial reaction in O-linked (mucin-type) oligosaccharide biosynthesis. It creates four isoforms: isoform 1: 603 AA long, nearly 69 kDA; isoform 2: 541 AA long; nearly 62 kDA; isoform 3: 366 AA long; 41 kDA; and isoform 4: 276 AA long: approximately 31 kDA. The GALNT10 gene has been studied with its contribution to thyroiditis. It interacts with genes MUC7, MUC1, GALNT1, GALNT12, and PTCH1 in the metabolism of proteins, mucin type O-glycan biosynthesis, and in post-translational protein modification.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GALNT10