![Immunohistochemistry-Paraffin: PIWIL3 Antibody [NBP2-31855] - Staining of human stomach, upper shows strong membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/PIWIL3-Antibody-Immunohistochemistry-Paraffin-NBP2-31855-img0007.jpg "Immunohistochemistry-Paraffin: PIWIL3 Antibody [NBP2-31855] - Staining of human stomach, upper shows strong membranous positivity in glandular cells.")
| Immunogen | This antibody was developed against a recombinant protein corresponding to amino acids: SRTSHREAMSLKGHLQSVTAPMGITMKPAEMIEVDGDANSYIDTLRKYTRPTLQMVICILPNDDKRRYDSI |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | PIWIL3 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | For IHC-Paraffin, HIER pH 6 retrieval is recommended. Use in WB reported in reported in scientific literature (PMID: 30701019). In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) and 40% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Immunogen affinity purified |
| Publication using NBP2-31855 | Applications | Species |
|---|---|---|
| Jacobs DI, Qin Q, Fu A et al. piRNA-8041 is downregulated in human glioblastoma and suppresses tumor growth in vitro and in vivo. Oncotarget 2018-12-28 [PMID: 30701019] (WB, Human) | WB | Human |
Secondary Antibodies |
Isotype Controls |
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![Simple Western: PIWIL3 Antibody [NBP2-31855] - Simple Western lane view shows a specific band for PIWIL3 in 0.2 mg/ml of RT-4 (Left) and U-251MG (Right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/PIWIL3-Antibody-Simple-Western-NBP2-31855-img0005.jpg "Simple Western: PIWIL3 Antibody [NBP2-31855] - Simple Western lane view shows a specific band for PIWIL3 in 0.2 mg/ml of RT-4 (Left) and U-251MG (Right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.")
![Simple Western: PIWIL3 Antibody [NBP2-31855] - Electropherogram image(s) of corresponding Simple Western lane view. PIWIL3 antibody was used at 1:20 dilution on RT-4 and U-251MG lysate(s).](http://images.novusbio.com/fullsize/PIWIL3-Antibody-Simple-Western-NBP2-31855-img0006.jpg "Simple Western: PIWIL3 Antibody [NBP2-31855] - Electropherogram image(s) of corresponding Simple Western lane view. PIWIL3 antibody was used at 1:20 dilution on RT-4 and U-251MG lysate(s).")
