Ovalbumin Antibody [Biotin]

Images

 
Western Blot: Ovalbumin Antibody [Biotin] [NB600-924] - Lane 1: Ovalbumin. Lane 2: None. Load: 50 ng per lane. Primary antibody: Ovalbumin biotin conjugated primary antibody at 1:1,000 for 60 min at RT. Secondary ...read more
Western Blot: Ovalbumin Antibody [Biotin] [NB600-924] - Lane 1: ~1 ug Ovalbumin protein reduced. Lane 2: 0.25 ug Ovalbumin protein reduced. Lane 3: ~1 ug Ovalbumin protein non-reduced. Lane 4: 0.25 ug Ovalbumin protein ...read more

Product Details

Summary
Reactivity Ch, V-ViSpecies Glossary
Applications WB, ELISA, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Biotin
Concentration
LYOPH

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Catalog# & Conjugate Size Price

Ovalbumin Antibody [Biotin] Summary

Description
This antibody is purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
Immunogen
Ovalbumin [Hen Egg White] (Uniprot: P01012)
Localization
Secreted
Specificity
Reacts with ovalbumin.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
OVAL
Purity
Multi-step
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:30000
  • Immunoprecipitation 1:100
  • Western Blot 1:500-1:5000
Application Notes
This antibody Biotin Conjugated is suitable for both ELISA, and for western blotting. In western blots anti-ovalbumin detects a single band of the expected apparent molecular weight. Researchers should determine optimal titers for applications that are not stated below.
Readout System
Publications
Read Publication using NB600-924.

Reactivity Notes

Cross reactivity against Ovalbumin from other sources is unknown Use in Virus reported in scientific literature (PMID:20451987).

Packaging, Storage & Formulations

Storage
Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Sodium Azide
Concentration
LYOPH
Purity
Multi-step
Reconstitution Instructions
Reconstitute with 100 ul deionized water (or equivalent)

Notes

This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Ovalbumin [Hen Egg White]

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Alternate Names for Ovalbumin Antibody [Biotin]

  • Allergen Gal d 2
  • CAP 2
  • CAP2
  • Cytoplasmic antiproteinase 2
  • Egg albumin
  • Gal d II
  • Hen egg white
  • PI8
  • Plakalbumin
  • Protease inhibitor 8
  • Serpin B8
  • serpin peptidase inhibitor clade B member 8
  • SERPINB14
  • SERPINB8

Background

Chicken Ovalbumin is the major protein in the "white" of the egg (and a favorite antigen in immunological research). Egg white contains a variety of proteins including ovalbumin, conalbumin, ovomucoid and lysozyme. It belongs to the serpin family and the Ov serpin subfamily. Ovalbumin can cause an allergic reaction in humans. Ovalbumin has been implicated in the development of the egg shell. Immunohistochemistry revealed that ovalbumin is found only in the mammillary bodies of decalcified shell, and is not distributed throughout the shell matrix. These results indicate that ovalbumin is present during the initial phase of shell formation and becomes incorporated into the protein matrix of the mammillary bodies. However, it is not yet clear whether ovalbumin at this site plays a specific role in shell mineralisation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Ovalbumin Antibody (NB600-924)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin NB600-924
HRP NB120-20546

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Bioinformatics

Gene Symbol OVAL