NMNAT3 Antibody

Western Blot: NMNAT3 Antibody [NBP2-27543] - NBP2-27543 (0.1ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

NMNAT3 Antibody Summary

• Description: Novus Biologicals Goat NMNAT3 Antibody (NBP2-27543) is a polyclonal antibody validated for use in WB and ELISA. Anti-NMNAT3 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-QHNIHLAKEPVQNE corresponding to internal region according to NP_835471.1, NP_001186976.1.
• Specificity: This antibody is expected to recognize both reported isoforms (NP_835471.1; NP_001186976.1).
• Predicted Species: Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: NMNAT3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.1 - 0.3 ug/ml
• Application Notes: WB: Approx. 26 kDa band observed in human kidney lysates (calculated MW of 24.1 kDa band according to NP_835471.1).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for NMNAT3 Antibody

• EC 2.7.7.1
• EC 2.7.7.18
• NaMN adenylyltransferase 3
• nicotinamide mononucleotide adenylyltransferase 3
• nicotinamide nucleotide adenylyltransferase 3
• Nicotinate-nucleotide adenylyltransferase 3
• PNAT-3
• PNAT3NMN adenylyltransferase 3
• Pyridine nucleotide adenylyltransferase 3

Background

The coenzyme NAD and its derivatives are involved in hundreds of metabolic redox reactions and are utilized in protein ADP-ribosylation, histone deacetylation, and in some Ca(2+) signaling pathways. NMNAT (EC 2.7.7.1) is a central enzyme in NAD biosynthesis, catalyzing the condensation of nicotinamide mononucleotide (NMN) or nicotinic acid mononucleotide (NaMN) with the AMP moiety of ATP to form NAD or NaAD (Zhang et al., 2003 [PubMed 12574164]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NMNAT3 Antibody (NBP2-27543)(1)

Publication using NBP2-27543

• Sorci L, Cimadamore F, Scotti S et al. Initial-rate kinetics of human NMN-adenylyltransferases: substrate and metal ion specificity, inhibition by products and multisubstrateanalogues, and isozyme contributions to NAD+ biosynthesis. Biochemistry 2007-04-24 [PMID: 17402747]

Bioinformatics

• Gene Symbol: NMNAT3
• Uniprot:
  • NP_835471.1(Human)