Mitochondria Antibody (113-1) [Biotin]

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Immunohistochemistry-Paraffin: Mitochondria Antibody (113-1) [Biotin] [NBP2-34517B] - Mitochondria expression in human prostate cancer tissue (left) and mouse stomach tissue (right). Image from verified customer review.

Product Details

Summary
Reactivity Hu, Mu(-), Rt(-)Species Glossary
Applications WB, ICC/IF, IHC-Fr, IHC-P
Clone
113-1
Clonality
Monoclonal
Host
Mouse
Conjugate
Biotin

Mitochondria Antibody (113-1) [Biotin] Summary

Immunogen
Semi-purified mitochondrial preparation
Localization
Mitochondria in cytoplasm
Marker
Marker for Human Cells, Granular RCC & Salivary Tumors
Specificity
This MAb recognizes a 60kDa antigen associated with the mitochondria in human cells. It can be used to stain mitochondria in cell or tissue preparations and can be used as a mitochondrial marker in subcellular fractions. It produces a spaghetti-like pattern in normal and malignant cells. This antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells, including neurons and embryonic stem cells. Immunostaining pattern with anti-mitochondrial MAb has been reported as a useful discriminatory adjunct in the complex differential diagnosis of granular renal cell tumors. Reportedly, this MAb facilitates the classification of salivary tumors.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Western Blot
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
Theoretical MW
60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Readout System
Reviewed Applications
Read 1 Review rated 5
using
NBP2-34517B in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Mitochondria Antibody (NBP2-34517B) (0)

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Review for Mitochondria Antibody (NBP2-34517B) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Other.
We have 1 review tested in 1 application: IHC-P.

Reviews using NBP2-34517B:
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Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin Mitochondria NBP2-34517B
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reviewed by:
Naiche Adler
IHC-P Other 03/26/2015
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedhuman prostate cancer, mouse stomach
SpeciesOther

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Mitochondria Antibody (NBP2-34517B) (0)

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Secondary Antibodies

 

Isotype Controls

Additional Mitochondria Products

Mitochondria NBP2-34517B

Blogs on Mitochondria.

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By Christina Towers, PhDA critical mechanism that cells use to generate nutrients and fuel metabolism is through a process called autophagy.  This process is complex and involves over 20 different proteins, most of which are highly conserved acro...  Read full blog post.

Dynamin-related Protein 1 (DRP1) in Mitochondria and Apoptosis.
Dynamin-related Protein 1 (DRP1) is known to function in mitochondrial and peroxisomal division and mediate membrane fission through oligomerization into ring-like structure and sever the mitochondrial membrane, through a GTP hydrolysis-dependent mech...  Read full blog post.

The Importance of the COX IV Antibody to Apoptosis Research
COX IV isoform 1 is a nuclear-encoded polypeptide chain of the Cytochrome C Oxidase enzyme, located on the mitochondrial inner membrane. Owing to its widespread distribution in human and mammalian tissues, COX IV antibodies are widely used as loading...  Read full blog post.

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Recent Reviews

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Naiche Adler
03/26/2015
Application: IHC-P
Species: Other