Western Blot: Mitochondria Antibody (113-1) [NBP2-32980] - Western Blot analysis of HeLa cell lysate using Mitochondria Antibody (113-1).
Immunocytochemistry/ Immunofluorescence: Mitochondria Antibody (113-1) [NBP2-32980] - Confocal immunofluorescence image of HeLa cells using labeled is Green (CF488) and Red Dot is used to label the nuclei Red.
Immunohistochemistry-Paraffin: Mitochondria Antibody (113-1) [NBP2-32980] - Immunohistochemistry-Paraffin: Mitochondria Antibody (113-1) - Azide and BSA Free [NBP2-34517] - Formalin-fixed paraffin-embedded human Renal ...read more
Immunohistochemistry-Paraffin: Mitochondria Antibody (113-1) [NBP2-32980] - Analysis using the Biotin conjugate of NBP2-32980. Staining of human prostate cancer tissue (left) and mouse stomach tissue (right). Image from ...read more
Immunohistochemistry-Paraffin: Mitochondria Antibody (113-1) [NBP2-32980] - Formalin-fixed, paraffin-embedded human testicular carcinoma stained with mitochondria monoclonal antibody (113-1). Image using the Azide and ...read more
Immunohistochemistry-Paraffin: Mitochondria Antibody (113-1) [NBP2-32980] - Formalin-fixed paraffin-embedded human pancreas stained with mitochondria monoclonal antibody (113-1). Image using the Azide and BSA Free form ...read more
Marker for Human Cells, Granular RCC & Salivary Tumors
This MAb recognizes a 60kDa antigen associated with the mitochondria in human cells. It can be used to stain mitochondria in cell or tissue preparations and can be used as a mitochondrial marker in subcellular fractions. It produces a spaghetti-like pattern in normal and malignant cells. This antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells, including neurons and embryonic stem cells. Immunostaining pattern with anti-mitochondrial MAb has been reported as a useful discriminatory adjunct in the complex differential diagnosis of granular renal cell tumors. Reportedly, this MAb facilitates the classification of salivary tumors.
Protein A or G purified
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Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues is enhanced by heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.
60 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publication using NBP2-32980 in the following applications:
There's an autophagy for that! By Christina Towers, PhDA critical mechanism that cells use to generate nutrients and fuel metabolism is through a process called autophagy. This process is complex and involves over 20 different proteins, most of which are highly conserved acro... Read full blog post.
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