MFSD1 Knockout HeLa Cell Lysate

Product Discontinued

MFSD1 Knockout HeLa Cell Lysate Summary

• Preparation Method: Knockout achieved by using CRISPR/Cas9,1 bp deletion in exon 1
• Gene: MFSD1

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: You will receive 1 vial (100ug) of knockout cell lysate and 1 vial (100ug) of Parental cell lysate. Lysate can be diluted with 1X SDS sample buffer and will be stable at -20 degrees C for 12 months. Minimize freeze-thaw cycles.

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 0.1 mg cell homogenate lyophilized in RIPA buffer made with double-knockout cell lines.
• Concentration: LYOPH
• Reconstitution Instructions: To use as WB negative control, spin down briefly and resuspend in 100 uL 1xSDS sample buffer (2% SDS, 60 mM Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol). Boil the lysate for 3 - 5 minutes before loading it onto gel.

Lysate Details for Array

• Type: Knockout HeLa Cell

Notes

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Validation of antibody specificity is critical and verification of antibody performance against knockout samples is one way to guarantee that an antibody recognizes a specific target. Novus' KO cell lysate can be used as a negative control for western blots and to confirm the specificity of antibodies.

Alternate Names for MFSD1 Knockout HeLa Cell Lysate

• FLJ14153
• major facilitator superfamily domain containing 1
• major facilitator superfamily domain-containing protein 1
• SMAP4
• SMAP-4
• Smooth muscle cell-associated protein 4
• UG0581B09

Background

The MFSD1 gene encodes a major facilitator superfamily domain-containing protein 1 that exists in four isoforms: isoform 1: 465 amino acids long, 51 kDA; isoform 2: 78 amino acids long, 8 kDA; isoform 3: 426 amino acids long, 46 kDA; and isoform 4: 392 amino acids long, 43 kDA. MFSD1 interacts with genes UBC, TMEM33, RAB10, and TOMM70A. This gene has been linked to salivary gland cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: MFSD1