MFAP3 Antibody (1059143) [Allophycocyanin]

HEK293 cells transfected with Human MFAP-3L and eGFP were stained with eGFP and either (A) Mouse Anti-Human MFAP3 APC‑conjugated Monoclonal Antibody (Catalog # FAB113251A) or (B) Mouse IgG2B Allophycocyanin Isotype Control (Catalog # IC0041A). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 1059143
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Allophycocyanin

MFAP3 Antibody (1059143) [Allophycocyanin] Summary

• Immunogen: Human embryonic kidney cell, HEK293-derived human MFAP3L
  Met1-Met149
  Accession # O75121
• Specificity: Detects human MFAP3L in direct ELISA.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MFAP3 Antibody (1059143) [Allophycocyanin]

• DKFZp586F0219
• MFAP3
• microfibril-associated glycoprotein 3
• microfibrillar-associated protein 3

Background

Microbibrillar-Associated Protein 3-Like (MFAP3L), also known as NYD-sp9, is part of the microfibrillar-associated protein family (MFAPs). MFAPs are non‐fibrillin, extracellular matrix glycoproteins that interact with fibrillin and were originally characterized in microfibrillar assembly (1, 2). In humans, there several subfamily members with varying amino acid (aa) sequence homology and functions (1, 2). Among the family, MFAP2 and MFAP5 are more closely related and while MFAP1, 3 and 4 share no structural or sequence homology with MFAP2, MFAP5 or with each other (1, 2). Human MFAP3L shows 71% amino acid (aa) sequence homology to MFAP3, but not other MFAPs (3). Mature, human MFAP3L consists of an extracellular domain (ECD) containing N-linked glycosylation sites, a transmembrane domain, and a cytoplasmic domain with a conserved SH2 motif (3). The ECD of human MFAP3L shares 89% and 90% aa sequence identity with mouse and rat MFAP3L, respectively. MFAPs have the unique ability to interact with TGF-beta family growth factors, Notch and Notch ligands and multiple elastic fiber proteins, in addition to interacting with fibrillin (1, 2). MFAPs are expressed in a wide variety of tissues and, along with microfibril assembly, they play roles in the regulation of tissue homeostasis, cell survival, and tumor progression (1, 2). MFAP3L is often located within colorectal cancer (CRC) cells, which metastasize by activation of the nuclear ERK pathway via MFAP3L phosphorylation (3). Regulation of this MFAP3L activity could have pharmaceutical effects on CRC tumor progression (3).

1. Zhu, S. et al. (2020) J Cell Physio. 236:41.
2. Mecham, R.P. et al. (2015) Matrix Biol. 47:13.
3. Lou, X. et al. (2014) BBA. 1842:1423.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • O75121()