| Reactivity | HuSpecies Glossary |
| Applications | PAGE |
| Concentration | 1 mg/ml |
| Description | A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-229 of Human METTL21D Source: E.coli Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMADTLES SLEDPLRSFV RVLEKRDGTV LRLQQYSSGG VGCVVWDAAI VLSKYLETPE FSGDGAHALS RRSVLELGSG TGAVGLMAAT LGADVVVTDL EELQDLLKMN INMNKHLVTG SVQAKVLKWG EEIEGFPSPP DFILMADCIY YEESLEPLLK TLKDISGFET CIICCYEQRT MGKNPEIEKK YFELLQLDFD FEKIPLEKHD EEYRSEDIHI IYIRKKKSKF PS |
| Source | E. coli |
| Protein/Peptide Type | Recombinant Protein |
| Gene | METTL21D |
| Purity | >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain |
| Theoretical MW | 28.2 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 20mM Phosphate buffer saline (pH 8.0) containing 10% glycerol, 1mM DTT |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain |
PTMs for METTL21D Recombinant Protein (NBP2-51911)Learn more about PTMs related to METTL21D Recombinant Protein (NBP2-51911).
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| Gene Symbol | METTL21D |
![SDS-Page: METTL21D Recombinant Protein [NBP2-51911] - 15% SDS Page (3 ug)](http://images.novusbio.com/fullsize2/METTL21D-Recombinant-Protein-SDS-Page-NBP2-51911-img0001.jpg "SDS-Page: METTL21D Recombinant Protein [NBP2-51911] - 15% SDS Page (3 ug)")
![Western Blot: p97/VCP Antibody [NB100-1557] - PC12 whole cell lysate, 30 ug total protein in each lane. Antibody at 1:1000. Image from verified customer review.](http://images.novusbio.com/fullsize2/p97-VCP-Antibody-Western-Blot-NB100-1557-img0008.jpg)
![Immunohistochemistry: p97/VCP Antibody [NB100-1557] - Sample: FFPE sections of human ovarian carcinoma (upper) and mouse squamous cell carcinoma (lower). Antibody: Affinity purified rabbit anti-VCP used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize2/p97-VCP-Antibody-Immunohistochemistry-NB100-1557-img0007.jpg)
![Western Blot: p97/VCP Antibody [NB100-1557] - Detection of Human and Mouse VCP on HeLa whole cell lysate using NB100-1557. VCP was also immunoprecipitated using rabbit anti-VCP antibodies NB100-1558 and Another VCP Ab at 3 mcg/mg lysate.](http://images.novusbio.com/fullsize2/p97-VCP-Antibody-Western-Blot-NB100-1557-img0001.jpg)
![Western Blot: Craniofacial Development Protein 1 Antibody (11811) [NBP2-43772] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CFDP1 antibody [11811].](http://images.novusbio.com/fullsize2/Craniofacial-Development-Protein-1-Antibody-(11811)-Western-Blot-NBP2-43772-img0003.jpg)
![Immunohistochemistry-Paraffin: Craniofacial Development Protein 1 Antibody (11811) [NBP2-43772] - Paraffin-embedded human ovarian cancer. CFDP1 stained by CFDP1 antibody [11811] diluted at 1:2000.](http://images.novusbio.com/fullsize2/Craniofacial-Development-Protein-1-Antibody-(11811)-Immunohistochemistry-Paraffin-NBP2-43772-img0005.jpg)
![Western Blot: Craniofacial Development Protein 1 Antibody (11811) [NBP2-43772] - Analysis of A U87-MG whole cell lysate/extract B SK-N-SH whole cell lysate/extract C IMR32 whole cell lysate/extract D SK-N-AS whole cell lysate/extract 12 % SDS-PAGE CFDP1 antibody [11811] dilution: 1:1000](http://images.novusbio.com/fullsize2/Craniofacial-Development-Protein-1-Antibody-(11811)-Western-Blot-NBP2-43772-img0001.jpg)
![Western Blot: Proteasome 19S 10B Antibody (1G6) [NBP2-00990] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Proteasome 19S 10B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Proteasome 19S 10B.](http://images.novusbio.com/fullsize2/Proteasome-19S-10B-Antibody-(1G6)-Western-Blot-NBP2-00990-img0019.jpg)
![Immunocytochemistry/Immunofluorescence: Proteasome 19S 10B Antibody (1G6) [NBP2-00990] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Proteasome 19S 10B.](http://images.novusbio.com/fullsize2/Proteasome-19S-10B-Antibody-(1G6)-Immunocytochemistry-Immunofluorescence-NBP2-00990-img0011.jpg)
![Immunohistochemistry-Paraffin: Proteasome 19S 10B Antibody (1G6) [NBP2-00990] - Staining of paraffin-embedded Human pancreas tissue using anti-Proteasome 19S 10B mouse monoclonal antibody.](http://images.novusbio.com/fullsize2/Proteasome-19S-10B-Antibody-(1G6)-Immunohistochemistry-Paraffin-NBP2-00990-img0018.jpg)
![Western Blot: DAP5 Antibody (39C534.1) [NB120-11929] - Analysis of NB120-11929 (1ug) on 15 ug of total cell lysate from Jurkat cells.](http://images.novusbio.com/fullsize2/DAP5 Antibody (39C534.1)-Western Blot-NB120-11929-img0003.jpg)
![Flow Cytometry: DAP5 Antibody (39C534.1) [NB120-11929] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.](http://images.novusbio.com/fullsize2/DAP5-Antibody-(39C534.1)-Flow-Cytometry-NB120-11929-img0007.jpg)
![Western Blot: Gemin 4 Antibody [NB110-40591] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. NB110-40591 used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate (B).](http://images.novusbio.com/fullsize2/Gemin-4-Antibody-Western-Blot-NB110-40591-img0003.jpg)
![Immunohistochemistry: Gemin 4 Antibody [NB110-40591] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-Gemin4 used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize2/Gemin 4 Antibody-Immunohistochemistry-NB110-40591-img0005.jpg)
![Immunohistochemistry-Paraffin: Gemin 4 Antibody [NB110-40591] - FFPE section of human breast carcinoma. Affinity purified rabbit anti-Gemin4 used at a dilution of 1:250.](http://images.novusbio.com/fullsize2/Gemin-4-Antibody-Immunohistochemistry-Paraffin-NB110-40591-img0001.jpg)
![Western Blot: p97/VCP Antibody [NB100-1557] - PC12 whole cell lysate, 30 ug total protein in each lane. Antibody at 1:1000. Image from verified customer review.](http://images.novusbio.com/images2/p97-VCP-Antibody-Western-Blot-NB100-1557-img0008.jpg "Western Blot: p97/VCP Antibody [NB100-1557] - PC12 whole cell lysate, 30 ug total protein in each lane. Antibody at 1:1000. Image from verified customer review.")
![Immunohistochemistry-Paraffin: METTL21D Antibody [NBP1-93526] - Staining of human breast shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/images2/METTL21D-Antibody-Immunohistochemistry-Paraffin-NBP1-93526-img0004.jpg "Immunohistochemistry-Paraffin: METTL21D Antibody [NBP1-93526] - Staining of human breast shows moderate cytoplasmic positivity in glandular cells.")
![Western Blot: Proteasome 19S 10B Antibody (1G6) [NBP2-00990] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Proteasome 19S 10B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Proteasome 19S 10B.](http://images.novusbio.com/images2/Proteasome-19S-10B-Antibody-(1G6)-Western-Blot-NBP2-00990-img0019.jpg "Western Blot: Proteasome 19S 10B Antibody (1G6) [NBP2-00990] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Proteasome 19S 10B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Proteasome 19S 10B.")