| Reactivity | HuSpecies Glossary |
| Applications | PAGE |
| Concentration | 1 mg/ml |
| Description | A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-229 of Human METTL21D Source: E.coli Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMADTLES SLEDPLRSFV RVLEKRDGTV LRLQQYSSGG VGCVVWDAAI VLSKYLETPE FSGDGAHALS RRSVLELGSG TGAVGLMAAT LGADVVVTDL EELQDLLKMN INMNKHLVTG SVQAKVLKWG EEIEGFPSPP DFILMADCIY YEESLEPLLK TLKDISGFET CIICCYEQRT MGKNPEIEKK YFELLQLDFD FEKIPLEKHD EEYRSEDIHI IYIRKKKSKF PS |
| Source | E. coli |
| Protein/Peptide Type | Recombinant Protein |
| Gene | METTL21D |
| Purity | >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain |
| Theoretical MW | 28.2 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 20mM Phosphate buffer saline (pH 8.0) containing 10% glycerol, 1mM DTT |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain |
PTMs for METTL21D Recombinant Protein (NBP2-51911)Learn more about PTMs related to METTL21D Recombinant Protein (NBP2-51911).
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| Gene Symbol | METTL21D |
![SDS-Page: METTL21D Recombinant Protein [NBP2-51911] - 15% SDS Page (3 ug)](http://images.novusbio.com/fullsize2/METTL21D-Recombinant-Protein-SDS-Page-NBP2-51911-img0001.jpg "SDS-Page: METTL21D Recombinant Protein [NBP2-51911] - 15% SDS Page (3 ug)")
![Western Blot: p97/VCP Antibody (5) [NB120-11433] - Analysis of CA46 cell lysate.](http://images.novusbio.com/fullsize2/p97-VCP-Antibody-(5)-Western-Blot-NB120-11433-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: p97/VCP Antibody (5) [NB120-11433] - Analysis of VCP using VCP Monoclonal antibody (5) shows staining in C6 glioma cells. VCP staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing VCP at a dilution of 1:20-1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize2/p97-VCP-Antibody-(5)-Immunocytochemistry-Immunofluorescence-NB120-11433-img0008.jpg)
![Immunohistochemistry-Paraffin: p97/VCP Antibody (5) [NB120-11433] - Both normal and cancer biopsies of deparaffinized Human colon carcinoma tissues.](http://images.novusbio.com/fullsize2/p97-VCP-Antibody-(5)-Immunohistochemistry-Paraffin-NB120-11433-img0003.jpg)
![Immunohistochemistry-Paraffin: Craniofacial Development Protein 1 Antibody [NBP1-88680] - Staining of human colon shows strong nuclear and cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize2/Craniofacial-Development-Protein-1-Antibody-Immunohistochemistry-Paraffin-NBP1-88680-img0002.jpg)
![Western Blot: Proteasome 19S 10B Antibody (1G6) [NBP2-00990] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Proteasome 19S 10B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Proteasome 19S 10B.](http://images.novusbio.com/fullsize2/Proteasome-19S-10B-Antibody-(1G6)-Western-Blot-NBP2-00990-img0019.jpg)
![Immunocytochemistry/Immunofluorescence: Proteasome 19S 10B Antibody (1G6) [NBP2-00990] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Proteasome 19S 10B.](http://images.novusbio.com/fullsize2/Proteasome-19S-10B-Antibody-(1G6)-Immunocytochemistry-Immunofluorescence-NBP2-00990-img0011.jpg)
![Immunohistochemistry-Paraffin: Proteasome 19S 10B Antibody (1G6) [NBP2-00990] - Staining of paraffin-embedded Human pancreas tissue using anti-Proteasome 19S 10B mouse monoclonal antibody.](http://images.novusbio.com/fullsize2/Proteasome-19S-10B-Antibody-(1G6)-Immunohistochemistry-Paraffin-NBP2-00990-img0018.jpg)
![Western Blot: DAP5 Antibody [NBP1-85311] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-EIF4G2 antibody. Remaining relative intensity is presented.](http://images.novusbio.com/fullsize2/DAP5-Antibody-Western-Blot-NBP1-85311-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: DAP5 Antibody [NBP1-85311] - Staining of human cell line U-2 OS shows positivity in cytoplasm.](http://images.novusbio.com/fullsize2/DAP5-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85311-img0004.jpg)
![Immunohistochemistry-Paraffin: DAP5 Antibody [NBP1-85311] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.](http://images.novusbio.com/fullsize2/DAP5-Antibody-Immunohistochemistry-Paraffin-NBP1-85311-img0001.jpg)
![Western Blot: Gemin 4 Antibody [NB110-40591] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. NB110-40591 used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate (B).](http://images.novusbio.com/fullsize2/Gemin-4-Antibody-Western-Blot-NB110-40591-img0003.jpg)
![Immunohistochemistry: Gemin 4 Antibody [NB110-40591] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-Gemin4 used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize2/Gemin 4 Antibody-Immunohistochemistry-NB110-40591-img0005.jpg)
![Immunohistochemistry-Paraffin: Gemin 4 Antibody [NB110-40591] - FFPE section of human breast carcinoma. Affinity purified rabbit anti-Gemin4 used at a dilution of 1:250.](http://images.novusbio.com/fullsize2/Gemin-4-Antibody-Immunohistochemistry-Paraffin-NB110-40591-img0001.jpg)
![Western Blot: p97/VCP Antibody (5) [NB120-11433] - Analysis of CA46 cell lysate.](http://images.novusbio.com/images2/p97-VCP-Antibody-(5)-Western-Blot-NB120-11433-img0004.jpg "Western Blot: p97/VCP Antibody (5) [NB120-11433] - Analysis of CA46 cell lysate.")
![Immunocytochemistry/Immunofluorescence: METTL21D Antibody [NBP1-93526] - Staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli and cytoplasm.](http://images.novusbio.com/images2/METTL21D-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-93526-img0003.jpg "Immunocytochemistry/Immunofluorescence: METTL21D Antibody [NBP1-93526] - Staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli and cytoplasm.")
![Western Blot: Proteasome 19S 10B Antibody (1G6) [NBP2-00990] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Proteasome 19S 10B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Proteasome 19S 10B.](http://images.novusbio.com/images2/Proteasome-19S-10B-Antibody-(1G6)-Western-Blot-NBP2-00990-img0019.jpg "Western Blot: Proteasome 19S 10B Antibody (1G6) [NBP2-00990] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Proteasome 19S 10B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Proteasome 19S 10B.")