Methionine Aminopeptidase 1D/MAP1D Antibody

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Methionine Aminopeptidase 1D/MAP1D Antibody Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Methionine Aminopeptidase 1D/MAP1D
  Arg44-Ala335
  Accession # Q6UB28
• Specificity: Detects human Methionine Aminopeptidase 1D/MAP1D in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: METAP1D
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 25 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Methionine Aminopeptidase 1D/MAP1D Antibody

• EC 3.4.11.18
• MAP1D
• MAP1DCDS of metAP-3 within PCR fragment
• MetAP1D
• Metap1l
• Methionine Aminopeptidase 1D
• methionine aminopeptidase 1D, mitochondrial
• methionyl aminopeptidase type 1D (mitochondrial)
• Methionyl aminopeptidase type 1D, mitochondrial

Background

Methionine Aminopeptidase 1D (MAP1D) is a member of the Methionine Aminopeptidase family (MetAPs) (1). MetAPs catalyze the removal of the initiator methionine residue from nascent peptides (2) and are essential for cell growth (3). MAP1D is localized in mitochondria (4) and is over-expressed in colon cancer cell lines and colon tumors (1). The N-terminal leader peptide (residue 1‑43) of MAP1D has been shown to be responsible for targeting to mitochondria (4).

1. Leszczyniecka, M. et al. (2006) Oncogene 25:3471.
2. Lowther, W.T. and B.W. Matthews (2000) Biochim. Biophys. Acta. 1477:157.
3. Li, X. and Y.H. Chang (1995) Proc. Natl. Acad. Sci. USA 92:12357.
4. Serero, A. et al. (2003) J. Biol. Chem. 278:52953.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: METAP1D
• Uniprot:
  • Q6UB28()