Melatonin Receptor 1B Overexpression Lysate

Product Discontinued

Melatonin Receptor 1B Overexpression Lysate Summary

• Immunogen: The lysate was created in HEK293T cells, using plasmid ID RC215316 and based on accession number NP_005950. The protein contains a C-terminal DDK tag.
• Specificity: Homo sapiens Melatonin Receptor 1B, mRNA.
• Gene: MTNR1B

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: This product is intended for use as a positive control in Western Blot.
  
  You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
• Theoretical MW: 40 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: RIPA buffer

Lysate Details for Array

• Type: Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Melatonin Receptor 1B Overexpression Lysate

• FGQTL2
• Mel 1b melatonin receptor
• Mel1b receptor
• MEL-1B-R
• melatonin receptor 1B
• melatonin receptor MEL1B
• melatonin receptor type 1B
• MT2

Background

MTNR1b is a Melatonin Receptor that mediates the effects of melatonin in the retina and the photoperiodic control of neuroendocrine functions. It may also be involved in melatonin-induced immune enhancement. MTNR1b has been reported in adipose, brain, eye, and ovary. An EST has been isolated from an eye tissue library.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

FAQs for Melatonin Receptor 1B Lysate (NBP2-04305). (Showing 1 - 1 of 1 FAQ).

1. I saw that you have the antibodies for the both receptors of melatonin but not listed with FACS application. I need more information about what is better for my assay.
   • At this time, none of our melatonin receptor antibodies have been tested and validated in Flow. Should you choose to purchase and test one of them, you would qualify for the Innovator's Rewards program. You should take a look at each individual antibody, the clonality, conjugates, immunogen region and all relevant data in order to decide which product would be most suitable for your needs.

Bioinformatics

• Gene Symbol: MTNR1B