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![Western Blot: Luciferase Antibody (Luci 21 1-107) [NB600-307] - Reduction in CREB activity in flies following DN-CREB expression in the fat body. CRE-Luciferase reporter protein was measured using anti-luciferase antibody in Western blots of body extracts from control flies (to-GAL4 driver only, control) or flies expressing DN-CREB in the fat body from the to-GAL4 driver (DN-CREB) (top panel). Blots were stripped and reprobed with anti-tubulin antibodies as a protein loading control (bottom panel). Signal intensities were quantified and are shown as ratios to control signals (mean+/-SD, n = 5; *p<0.05, Student's t-test). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0008498) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Luciferase-Antibody-Luci-21-1-107-Western-Blot-NB600-307-img0006.jpg "Western Blot: Luciferase Antibody (Luci 21 1-107) [NB600-307] - Reduction in CREB activity in flies following DN-CREB expression in the fat body. CRE-Luciferase reporter protein was measured using anti-luciferase antibody in Western blots of body extracts from control flies (to-GAL4 driver only, control) or flies expressing DN-CREB in the fat body from the to-GAL4 driver (DN-CREB) (top panel). Blots were stripped and reprobed with anti-tubulin antibodies as a protein loading control (bottom panel). Signal intensities were quantified and are shown as ratios to control signals (mean+/-SD, n = 5; *p<0.05, Student's t-test). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0008498) licensed under a CC-BY license.")
![Immunohistochemistry: Luciferase Antibody (Luci 21 1-107) [NB600-307] - Detection of Luciferase expression in CD34+ cells by Immunohistochemistry. Cytospin slides prepared from transduced CD34+ cells after 3 days of culture were stained with monoclonal anti-Luciferase antibody. Luciferase-positive cells have green cytoplasm; nuclei stained with DAPI are blue. Nontransduced, cultured CD34+ cells were used as a negative control. Original magnification, 40X. Wang, X. et al., Dynamic tracking of human hematopoietic stem cell. Blood. 102(10): 3478-3482, 2003.](http://images.novusbio.com/fullsize/Luciferase-Antibody-Luci-21-1-107-Immunohistochemistry-NB600-307-img0004.jpg)
![Western Blot: Luciferase Antibody (Luci 21 1-107) [NB600-307] - WB analysis of NB600-307 on Luciferase Protein (NB810-74573)](http://images.novusbio.com/fullsize/Luciferase-Antibody-Luci-21-1-107-Western-Blot-NB600-307-img0005.jpg)
![Western Blot: Luciferase Antibody (Luci 21 1-107) - BSA Free [NB600-307] - TTFields application leads to increased autophagic flux.a Ultra-structural STEM electron microscopy analysis of U-87 MG (upper panel) & A172 (lower panel) cells treated with TTFields for 48 h. Autophagosomes (blue arrows) & autolysosomes (green arrows) are indicated. b U-87 MG & A172 cells were either left untreated or were treated with TTFields for 24–72 h. CQ (20 µM) was added 4 h before cells were collected. Samples were immunoblotted for LC3 & GAPDH. Upper panel: representative blots. Lower panel: densitometric quantification of immunoblot signal, showing an average of at least three independent experiments (0.01 < *P < 0.05, Student’s t-test). c U-87 MG & A172 cells were either left untreated or were treated with TTFields for 48 h. CQ (20 µM) was added at the last 3 h of treatment & cells were fixed & stained with anti-LC3 Ab (green) & DAPI (blue). Upper panel: representative images. Original magnifications: × 40. Lower panel: quantification of LC3 intensity, presented as average intensity per cell (**P < 0.01, Student’s t-test). d U-87 MG cells were either left untreated or were treated with TTFields (48 h) or with vinblastin 25 nM (0.5 h). CQ (20 µM) was added at the last 3 h of treatment & cells were fixed & stained with anti-LC3 (green), LAMP1 (red), & DAPI (blue) (upper panel). Arrowheads indicate the strongest colocalization staining in each cell. Intensity histograms of LAMP1 & LC3 fluorescent signal calculated from the region of interest indicated by the white bar (lower panel). Representative images are shown Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30341282), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-307_mouse-monoclonal-luciferase-antibody-luci-21-1-107-310202416171421.jpg)
![Western Blot: Luciferase Antibody (Luci 21 1-107) - BSA Free [NB600-307] - Induction of autophagy by TTFields is AMPK dependent.a U-87 MG & A172 cells either left untreated or treated w/ TTFields for indicated time points. Immunoblot analysis of GFP78 protein. Numeric values represent fold increase in GRP78 signal, normalized to loading control (GAPDH), relative to untreated control. b Quantification of intracellular ATP levels in U-87 MG cells either left untreated or treated w/ TTFields for 72 h.Results presented as average ATP concentration (nmol/2 × 106 cells) from 3 independent experiments (*P < 0.01, Student’s t-test). c U-87 MG & A172 cells either left untreated or treated w/ TTFields for indicated time points. Immunoblot analysis of pAMPK & pULK1proteins. GAPDH used as loading control. (5D-F) U-87 MG cells transfected w/ AMPK-targeting siRNA (siAMPK) or w/ siRNA sham vector (siVector), & incubated for 48 h w/ or w/out TTFields application. CQ 20 µM added for last 4 h of treatment where indicated. d (left panel) Immunoblot analysis of LC3 & AMPK. Numeric values represent fold-change in LC3-II signal, normalized to GAPDH signal, relative to respective control. d (right panel) CQ-treated cells fixed & stained for LC3 (green) & DAPI (blue), original magnifications: × 40. e Cell count of siAMPK- or siVector-expressing cells after TTFields treatment. (0.01 < *P < 0.05, Student’s t-test, n = 3). f siVector- & siAMPK-transfected U-87 MG cells either left untreated or treated w/ TTFields for 48 h. Cells then fixed & stained for cleaved caspase-3 (green) & DAPI (blue) (left panel). Images from each treatment analyzed manually & fraction of cleaved caspase-3-positive cells calculated for at least 200 cells from each group (right panel) (**P < 0.01, Student’s t-test, n = 2) Image collected & cropped by CiteAb from following publication (//pubmed.ncbi.nlm.nih.gov/30341282), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-307_mouse-monoclonal-luciferase-antibody-luci-21-1-107-31020241616016.jpg)
![Western Blot: Luciferase Antibody (Luci 21 1-107) - BSA Free [NB600-307] - TTFields induce autophagy in glioma cell lines.a U-87 MG & A172 cells were either left untreated or treated with TTFields at the last 24 h, 48 h, or 72 h of culturing. All cultures were plated on the same time, incubated overnight to allow cell attachment, & collected 72 h afterwards. Cells were collected, lysed, & samples were analyzed using immunoblotting for LC3 & GAPDH. Upper panel: representative blots. Lower panel: densitometric quantification of immunoblot signal, showing an average of at least three independent experiments (0.01 < *P < 0.05, **P < 0.01, Student’s t-test). b Paraffin-embedded sections from sham- or TTFields-treated rats were stained with anti-LC3 Ab (green) & DAPI (blue). Representative images are presented. c Quantification of LC3 intensity, presented as fold increase from corresponding control (*P < 0.05, Student’s t-test) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30341282), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-307_mouse-monoclonal-luciferase-antibody-luci-21-1-107-310202416163711.jpg)
![Immunocytochemistry/ Immunofluorescence: Luciferase Antibody (Luci 21 1-107) - BSA Free [NB600-307] - Immunofluorescence imaging of luciferase protein as a reporter of hybrid formation. Entire primary tumor & lung sections were imaged via tile scanning, & each image of the scan was carefully analyzed to confirm or refute positive staining for luciferase. The luciferase signal was considered a positive signal if it was above background levels associated with negative controls & corresponded to the cytoplasm of a cell with a nucleus. Rare luciferase-positive cells were detected in the primary tumors. Most red signal was not in the cytoplasm of cells associated with nuclei and, therefore, considered nonspecific [insets (a), (c)]. The lungs containing metastases on the other hand [(b), (d)] contained a large number of bona fide luciferase-positive cells corresponding to fusion products. Scale bars on slide scans = 100 μm. Scale bars on 40× inset = 25 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31069316), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-307_mouse-monoclonal-luciferase-antibody-luci-21-1-107-31020241618921.jpg)
![Western Blot: Luciferase Antibody (Luci 21 1-107) - BSA Free [NB600-307] - TTFields induce autophagy in glioma cell lines.a U-87 MG & A172 cells were either left untreated or treated with TTFields at the last 24 h, 48 h, or 72 h of culturing. All cultures were plated on the same time, incubated overnight to allow cell attachment, & collected 72 h afterwards. Cells were collected, lysed, & samples were analyzed using immunoblotting for LC3 & GAPDH. Upper panel: representative blots. Lower panel: densitometric quantification of immunoblot signal, showing an average of at least three independent experiments (0.01 < *P < 0.05, **P < 0.01, Student’s t-test). b Paraffin-embedded sections from sham- or TTFields-treated rats were stained with anti-LC3 Ab (green) & DAPI (blue). Representative images are presented. c Quantification of LC3 intensity, presented as fold increase from corresponding control (*P < 0.05, Student’s t-test) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30341282), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-307_mouse-monoclonal-luciferase-antibody-luci-21-1-107-310202416163757.jpg)
![Immunocytochemistry/ Immunofluorescence: Luciferase Antibody (Luci 21 1-107) - BSA Free [NB600-307] - TTFields application leads to increased autophagic flux.a Ultra-structural STEM electron microscopy analysis of U-87 MG (upper panel) & A172 (lower panel) cells treated with TTFields for 48 h. Autophagosomes (blue arrows) & autolysosomes (green arrows) are indicated. b U-87 MG & A172 cells were either left untreated or were treated with TTFields for 24–72 h. CQ (20 µM) was added 4 h before cells were collected. Samples were immunoblotted for LC3 & GAPDH. Upper panel: representative blots. Lower panel: densitometric quantification of immunoblot signal, showing an average of at least three independent experiments (0.01 < *P < 0.05, Student’s t-test). c U-87 MG & A172 cells were either left untreated or were treated with TTFields for 48 h. CQ (20 µM) was added at the last 3 h of treatment & cells were fixed & stained with anti-LC3 Ab (green) & DAPI (blue). Upper panel: representative images. Original magnifications: × 40. Lower panel: quantification of LC3 intensity, presented as average intensity per cell (**P < 0.01, Student’s t-test). d U-87 MG cells were either left untreated or were treated with TTFields (48 h) or with vinblastin 25 nM (0.5 h). CQ (20 µM) was added at the last 3 h of treatment & cells were fixed & stained with anti-LC3 (green), LAMP1 (red), & DAPI (blue) (upper panel). Arrowheads indicate the strongest colocalization staining in each cell. Intensity histograms of LAMP1 & LC3 fluorescent signal calculated from the region of interest indicated by the white bar (lower panel). Representative images are shown Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30341282), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-307_mouse-monoclonal-luciferase-antibody-luci-21-1-107-31020241616033.jpg)
![Immunocytochemistry/ Immunofluorescence: Luciferase Antibody (Luci 21 1-107) - BSA Free [NB600-307] - TTFields application leads to increased autophagic flux.a Ultra-structural STEM electron microscopy analysis of U-87 MG (upper panel) & A172 (lower panel) cells treated with TTFields for 48 h. Autophagosomes (blue arrows) & autolysosomes (green arrows) are indicated. b U-87 MG & A172 cells were either left untreated or were treated with TTFields for 24–72 h. CQ (20 µM) was added 4 h before cells were collected. Samples were immunoblotted for LC3 & GAPDH. Upper panel: representative blots. Lower panel: densitometric quantification of immunoblot signal, showing an average of at least three independent experiments (0.01 < *P < 0.05, Student’s t-test). c U-87 MG & A172 cells were either left untreated or were treated with TTFields for 48 h. CQ (20 µM) was added at the last 3 h of treatment & cells were fixed & stained with anti-LC3 Ab (green) & DAPI (blue). Upper panel: representative images. Original magnifications: × 40. Lower panel: quantification of LC3 intensity, presented as average intensity per cell (**P < 0.01, Student’s t-test). d U-87 MG cells were either left untreated or were treated with TTFields (48 h) or with vinblastin 25 nM (0.5 h). CQ (20 µM) was added at the last 3 h of treatment & cells were fixed & stained with anti-LC3 (green), LAMP1 (red), & DAPI (blue) (upper panel). Arrowheads indicate the strongest colocalization staining in each cell. Intensity histograms of LAMP1 & LC3 fluorescent signal calculated from the region of interest indicated by the white bar (lower panel). Representative images are shown Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30341282), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-307_mouse-monoclonal-luciferase-antibody-luci-21-1-107-310202416175224.jpg)
![Dot Blot: Luciferase Antibody [NBP2-44165] - Dot Blot results of Rabbit anti-Luciferase Antibody. Dots are Luciferase (Photobacterium fischerii) at (1) 100ng, (2) 33.3ng, (3) 11.1ng, (4) 3.70ng, (5) 1.23ng. Primary Antibody: Rabbit anti-Luciferase. Secondary Antibody: Goat Anti-Rabbit IgG HRP.](http://images.novusbio.com/fullsize/Luciferase-Antibody-Dot-Blot-NBP2-44165-img0001.jpg "Dot Blot: Luciferase Antibody [NBP2-44165] - Dot Blot results of Rabbit anti-Luciferase Antibody. Dots are Luciferase (Photobacterium fischerii) at (1) 100ng, (2) 33.3ng, (3) 11.1ng, (4) 3.70ng, (5) 1.23ng. Primary Antibody: Rabbit anti-Luciferase. Secondary Antibody: Goat Anti-Rabbit IgG HRP.")
![Immunocytochemistry/Immunofluorescence: Luciferase Antibody (HL2164) - Azide and BSA Free [NBP3-25468] - Firefly Luciferase antibody [HL2164] detects Firefly Luciferase protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Firefly Luciferase stained by Firefly Luciferase antibody [HL2164] (NBP3-25468) diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25468_rabbit-luciferase-mab-hl2164-azide-and-bsa-free-2120249403896.jpg "Immunocytochemistry/Immunofluorescence: Luciferase Antibody (HL2164) - Azide and BSA Free [NBP3-25468] - Firefly Luciferase antibody [HL2164] detects Firefly Luciferase protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Firefly Luciferase stained by Firefly Luciferase antibody [HL2164] (NBP3-25468) diluted at 1:500. Blue: Fluoroshield with DAPI .")
![Western Blot: Luciferase Antibody (HL2164) - Azide and BSA Free [NBP3-25468] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Firefly Luciferase antibody [HL2164] (NBP3-25468) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25468_rabbit-luciferase-mab-hl2164-azide-and-bsa-free-2120249374961.jpg)
![SDS-Page: Recombinant Firefly Luciferase Protein [NBP2-59648] - 15% SDS-PAGE (3ug)](http://images.novusbio.com/fullsize/Recombinant-Firefly-Luciferase-Protein-SDS-Page-NBP2-59648-img0001.jpg "SDS-Page: Recombinant Firefly Luciferase Protein [NBP2-59648] - 15% SDS-PAGE (3ug)")
![Western Blot: Renilla Luciferase Antibody [NBP2-42914] - Renilla Luciferase antibody detects Renilla Luciferase protein by Western blot analysis. Non-transfected (-) and Renilla Luciferase -transfected (+, ) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Renilla Luciferase antibody at a dilution of 1:2000.](http://images.novusbio.com/fullsize/Renilla-Luciferase-Antibody-Western-Blot-NBP2-42914-img0001.jpg "Western Blot: Renilla Luciferase Antibody [NBP2-42914] - Renilla Luciferase antibody detects Renilla Luciferase protein by Western blot analysis. Non-transfected (-) and Renilla Luciferase -transfected (+, ) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Renilla Luciferase antibody at a dilution of 1:2000.")
![SDS-Page: Recombinant Luciferase (firefly) Protein [NBP3-18743] - 10% SDS-PAGE analysis](http://images.novusbio.com/fullsize/Recombinant-Luciferase-firefly-Protein-SDS-Page-NBP3-18743-img0001.jpg "SDS-Page: Recombinant Luciferase (firefly) Protein [NBP3-18743] - 10% SDS-PAGE analysis")
![Functional: Recombinant Luciferase (firefly) Protein [NBP3-18743] - An ATP dose response curve was performed in 50 ul reaction cocktails containing decreasing amounts of ATP, 0.2 ug Luciferase, and 15 nmol luciferin in a Tris-glycine buffer, pH 7.6 supplemented with 0.5 mM EDTA, 5 mM MgSO4 and 0.1% BSA. Luminescence was measured at 22C for 10 s in quadruplicates, using a GloMax 20/20 Luminometer.](http://images.novusbio.com/fullsize/Recombinant-Luciferase-firefly-Protein-Functional-NBP3-18743-img0002.jpg)
![Recombinant Luciferase (Firefly) Protein [NBP3-18743]—ATP is hydrolyzed, and light is emitted when firefly luciferase catalyzes the oxidation of D-luciferin. Reaction (1) is reversible, and the equilibrium lies far to the right. Reaction (2) is essentially irreversible. When ATP is the limiting reagent, the light emitted is proportional to the ATP present.](http://images.novusbio.com/fullsize/Recombinant-Luciferase-firefly-Protein-N-A-NBP3-18743-img0003.jpg)
![Western Blot: Renilla Luciferase Antibody [NBP2-42916] - Analysis of 30 ug whole cell lysate. A: 293T B: 293T transfected firefly C: 293T transfected Renilla Luciferase 10% SDS PAGE diluted at 1:1000.](http://images.novusbio.com/fullsize/Renilla-Luciferase-Antibody-Western-Blot-NBP2-42916-img0001.jpg "Western Blot: Renilla Luciferase Antibody [NBP2-42916] - Analysis of 30 ug whole cell lysate. A: 293T B: 293T transfected firefly C: 293T transfected Renilla Luciferase 10% SDS PAGE diluted at 1:1000.")
![Western Blot: Luciferase (firefly) Antibody [NBP3-33161] - Western Blot of Luciferase (firefly) Antibody. Lane 1: Luciferase (Firefly). Load: 50 ng per lane. Primary antibody: Luciferase (firefly) Antibody at 1:1,000 overnight at 4°C. Secondary antibody: HRP goat secondary antibody at 1:40,000 for 30 min at RT. Block for 30 min at RT. Predicted/Observed size: 60 kDa, 60 kDa for Luciferase.](http://images.novusbio.com/fullsize/nbp3-33161_goat-luciferase-firefly-pab-231202523584719.jpg "Western Blot: Luciferase (firefly) Antibody [NBP3-33161] - Western Blot of Luciferase (firefly) Antibody. Lane 1: Luciferase (Firefly). Load: 50 ng per lane. Primary antibody: Luciferase (firefly) Antibody at 1:1,000 overnight at 4°C. Secondary antibody: HRP goat secondary antibody at 1:40,000 for 30 min at RT. Block for 30 min at RT. Predicted/Observed size: 60 kDa, 60 kDa for Luciferase.")