WB, Flow, ICC/IF, IHC, IHC-PHost:
WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-PHost:
WB, IHC, IHC-FrHost:
Luciferase is a luminescent enzyme, mostly known for its role as a reporter protein. Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample.
- luciferin 4 monooxygenase
- ec 18.104.22.168