![Western Blot: Kos1/Tnk1 Antibody [NB300-237] - T47D cells expressing GFP/GFP-Kos were lysed in RIPA lysis buffer. 500ug protein from clarified cell lysates was immunoprecipitated with an anti-GFP antibody. The washed immunoprecipitates were electrophoresed on 8% SDS-PAGE gel and the proteins were transferred to nitrocellulose membrane. Panel A indicates GFP and GFP-Kos when probed with anti-GFP antibody. The blot was stripped thereafter, and GFP-Kos was detected with Kos1/Tnk1 antibody (NB300-237) at a dilution of 1:1000](http://images.novusbio.com/fullsize/Kos1-Tnk1-Antibody-Western-Blot-NB300-237-img0003.jpg "Western Blot: Kos1/Tnk1 Antibody [NB300-237] - T47D cells expressing GFP/GFP-Kos were lysed in RIPA lysis buffer. 500ug protein from clarified cell lysates was immunoprecipitated with an anti-GFP antibody. The washed immunoprecipitates were electrophoresed on 8% SDS-PAGE gel and the proteins were transferred to nitrocellulose membrane. Panel A indicates GFP and GFP-Kos when probed with anti-GFP antibody. The blot was stripped thereafter, and GFP-Kos was detected with Kos1/Tnk1 antibody (NB300-237) at a dilution of 1:1000")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, ICC/IF |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | C-terminal residues 421-434 [QNQVQSLPAGSAEP] of the 47 kDa mouse Kos1 / Tnk1 protein. |
| Specificity | This is specific for Kos1/Tnk1 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This antibody may be used for Western blot and immunocytochemistry/immunofluorescence. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
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![Immunocytochemistry/Immunofluorescence: Kos1/Tnk1 Antibody [NB300-237] - Kos1 staining in Hela cells detected with a Dylight 488 labeled secondary antibody.](http://images.novusbio.com/fullsize/Kos1-Tnk1-Antibody-Immunocytochemistry-Immunofluorescence-NB300-237-img0001.jpg "Immunocytochemistry/Immunofluorescence: Kos1/Tnk1 Antibody [NB300-237] - Kos1 staining in Hela cells detected with a Dylight 488 labeled secondary antibody.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")