Kos1/Tnk1 Antibody

Images

 
Western Blot: Kos1/Tnk1 Antibody [NB300-237] - T47D cells expressing GFP/GFP-Kos were lysed in RIPA lysis buffer. 500ug protein from clarified cell lysates was immunoprecipitated with an anti-GFP antibody. The washed ...read more
Immunocytochemistry/ Immunofluorescence: Kos1/Tnk1 Antibody [NB300-237] - Kos1 staining in Hela cells detected with a Dylight 488 labeled secondary antibody.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Kos1/Tnk1 Antibody Summary

Immunogen
C-terminal residues 421-434 [QNQVQSLPAGSAEP] of the 47 kDa mouse Kos1 / Tnk1 protein.
Specificity
This is specific for Kos1/Tnk1
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
Application Notes
This antibody may be used for Western blot and immunocytochemistry/immunofluorescence.

Reactivity Notes

Mouse and human.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Background

Kinase of embryonic stem cells (Kos1) is a nonreceptor protein tyrosine kinase (NRPTK) and a new member of the Ack family of NRPTKs. Kos1 is a ubiquitously expressed 47-kDa protein with autotyrosine kinase activity that is developmentally regulated during embryogenesis. Kos1 is also upregulated following IL3 withdrawal from factor-dependent murine NSF/N1.H7 cells that undergo apoptosis, suggesting a role in growth inhibition. Data support a negative regulatory role for Kos1 in regulating the Ras-Raf1-MAPK growth pathway by a mechanism that requires its autotyrosine kinase activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Entrez
Uniprot