![Sandwich ELISA: IgG4 Fc Antibody (RM217) - Azide and BSA Free [NBP3-25978] - Sandwich ELISA using RM217 as the capture antibody, and NBP3-18532 as the detection antibody, followed by an AP conjugated streptavidin.](http://images.novusbio.com/fullsize/nbp3-25978_rabbit-igg4-fc-mab-rm217-azide-and-bsa-free-22620242311069.png "Sandwich ELISA: IgG4 Fc Antibody (RM217) - Azide and BSA Free [NBP3-25978] - Sandwich ELISA using RM217 as the capture antibody, and NBP3-18532 as the detection antibody, followed by an AP conjugated streptavidin.")
| Reactivity | HuSpecies Glossary |
| Applications | ELISA, ELISA |
| Clone | RM217 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Biotin | NBP2-62127B-0.05mg | |||
| Unconjugated | NBP2-62127 | |||
| Description | Novus Biologicals Rabbit IgG4 Fc Antibody (RM217) - Azide and BSA Free (NBP2-62127) is a recombinant monoclonal antibody validated for use in ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Peptide corresponding to the Fc region of Human IgG4 |
| Specificity | This antibody reacts to the Fc region of human IgG4. No cross reactivity with human IgG1, IgG2, IgG3, IgM, IgA, IgD, IgE, mouse IgG, rat IgG, or goat IgG. |
| Source | HEK293 |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
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![Multiplex Immunoassay: IgG4 Fc Antibody (RM217) - Azide and BSA Free [NBP3-25978] - RM217 specifically reacts to human IgG4. No cross reactivity with human IgG1, IgG2, IgG3](http://images.novusbio.com/fullsize/nbp3-25978_rabbit-igg4-fc-mab-rm217-azide-and-bsa-free-24620241332438.png "Multiplex Immunoassay: IgG4 Fc Antibody (RM217) - Azide and BSA Free [NBP3-25978] - RM217 specifically reacts to human IgG4. No cross reactivity with human IgG1, IgG2, IgG3")
![ELISA: IgG4 Fc Antibody (RM217) [NBP3-25978] - Sandwich ELISA using biotinylated RM217 (NBP2-62127B) as the capture antibody, and biotinylated anti-human light chains (kappa + lambda) antibody as the detection antibody, followed by an AP conjugated streptavidin.](http://images.novusbio.com/fullsize/nbp3-25978_rabbit-igg4-fc-mab-rm217-azide-and-bsa-free-elisa-189202523374012.png "ELISA: IgG4 Fc Antibody (RM217) [NBP3-25978] - Sandwich ELISA using biotinylated RM217 (NBP2-62127B) as the capture antibody, and biotinylated anti-human light chains (kappa + lambda) antibody as the detection antibody, followed by an AP conjugated streptavidin.")
![Multiplex Immunoassay: IgG4 Fc Antibody (RM217) - Azide and BSA Free [NBP3-25978] - RM217 specifically reacts to human IgG4. No cross reactivity with human IgG1, IgG2, IgG3](http://images.novusbio.com/fullsize/nbp3-25978_rabbit-igg4-fc-mab-rm217-azide-and-bsa-free-multiplex-immunoassay-18920252342354.png "Multiplex Immunoassay: IgG4 Fc Antibody (RM217) - Azide and BSA Free [NBP3-25978] - RM217 specifically reacts to human IgG4. No cross reactivity with human IgG1, IgG2, IgG3")
![ELISA: IgG4 Fc Antibody (RM217) - Azide and BSA Free [NBP3-25978] - A titer ELISA using RM217. The plate was coated with different amounts of human IgG4. A serial dilution of RM217 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.](http://images.novusbio.com/fullsize/nbp3-25978_rabbit-igg4-fc-mab-rm217-azide-and-bsa-free-elisa-189202523415413.png "ELISA: IgG4 Fc Antibody (RM217) - Azide and BSA Free [NBP3-25978] - A titer ELISA using RM217. The plate was coated with different amounts of human IgG4. A serial dilution of RM217 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.")
![Sandwich ELISA: IgG4 Fc Antibody (RM217) - Azide and BSA Free [NBP3-25978] - Sandwich ELISA using RM217 as the capture antibody, and NBP3-18532 as the detection antibody, followed by an AP conjugated streptavidin.](http://images.novusbio.com/fullsize/nbp3-25978_rabbit-igg4-fc-mab-rm217-azide-and-bsa-free-sandwich-elisa-189202523362018.png "Sandwich ELISA: IgG4 Fc Antibody (RM217) - Azide and BSA Free [NBP3-25978] - Sandwich ELISA using RM217 as the capture antibody, and NBP3-18532 as the detection antibody, followed by an AP conjugated streptavidin.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")