![Sandwich ELISA: IgG4 Fc Antibody (RM217) - Azide and BSA Free [NBP2-62127] - Sandwich ELISA using RM217 as the capture antibody, and NBP3-18532 as the detection antibody, followed by an AP conjugated streptavidin.](http://images.novusbio.com/fullsize/nbp2-62127_rabbit-monoclonal-igg4-fc-antibody-rm217-sandwich-elisa-189202523394313.png "Sandwich ELISA: IgG4 Fc Antibody (RM217) - Azide and BSA Free [NBP2-62127] - Sandwich ELISA using RM217 as the capture antibody, and NBP3-18532 as the detection antibody, followed by an AP conjugated streptavidin.")
| Reactivity | HuSpecies Glossary |
| Applications | ELISA, ELISA |
| Clone | RM217 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Description | Novus Biologicals Rabbit IgG4 Fc Antibody (RM217) (NBP2-62127) is a recombinant monoclonal antibody validated for use in ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Additional Information | Recombinant Monoclonal Antibody. |
| Immunogen | Peptide corresponding to the Fc region of Human IgG4 |
| Specificity | This antibody reacts to the Fc region of human IgG4. No cross reactivity with human IgG1, IgG2, IgG3, IgM, IgA, IgD, IgE, mouse IgG, rat IgG, or goat IgG. |
| Source | HEK293 |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | 50% Glycerol/PBS, 1% BSA |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for IgG4 Fc Antibody (NBP2-62127)Find related products by research area.
|
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![ELISA: IgG4 Fc Antibody (RM217) [Biotin] [NBP2-62127] - Sandwich ELISA using NBP2-62127B as the capture antibody, and biotinylated anti-human light chains (kappa + lambda) antibody as the detection antibody, followed by an AP conjugated streptavidin.](http://images.novusbio.com/fullsize/nbp2-62127_rabbit-monoclonal-igg4-fc-antibody-rm217-elisa-189202523381717.png "ELISA: IgG4 Fc Antibody (RM217) [Biotin] [NBP2-62127] - Sandwich ELISA using NBP2-62127B as the capture antibody, and biotinylated anti-human light chains (kappa + lambda) antibody as the detection antibody, followed by an AP conjugated streptavidin.")
![ELISA: IgG4 Fc Antibody (RM217) - Azide and BSA Free [NBP2-62127] - A titer ELISA using RM217. The plate was coated with different amounts of human IgG4. A serial dilution of RM217 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.](http://images.novusbio.com/fullsize/nbp2-62127_rabbit-monoclonal-igg4-fc-antibody-rm217-elisa-18920252337124.png "ELISA: IgG4 Fc Antibody (RM217) - Azide and BSA Free [NBP2-62127] - A titer ELISA using RM217. The plate was coated with different amounts of human IgG4. A serial dilution of RM217 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.")
![Multiplex Immunoassay: IgG4 Fc Antibody (RM217) - Azide and BSA Free [NBP2-62127] - RM217 specifically reacts to human IgG4. No cross reactivity with human IgG1, IgG2, IgG3](http://images.novusbio.com/fullsize/nbp2-62127_rabbit-monoclonal-igg4-fc-antibody-rm217-multiplex-immunoassay-189202523423515.png "Multiplex Immunoassay: IgG4 Fc Antibody (RM217) - Azide and BSA Free [NBP2-62127] - RM217 specifically reacts to human IgG4. No cross reactivity with human IgG1, IgG2, IgG3")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")