IDH3G Antibody

Western Blot: IDH3G Antibody [NBP1-78786] - Analysis of Rat (A) and Pig (B) Heart lysate (35ug protein in RIPA buffer).

Western Blot: IDH3G Antibody [NBP1-78786] - Analysis of MCF7 lysate (35ug protein in RIPA buffer).

• Reactivity: Hu, Mu, Rt, Po, Bv, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

IDH3G Antibody Summary

• Immunogen: Peptide with sequence C-DHLKLHSYATSIRK corresponding to internal region according to NP_777358.1.
• Specificity: This antibody is expected to recognize both reported isoforms (NP_004126.1; NP_777358.1).
• Predicted Species: Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IDH3G
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 0.5 - 2 ug/ml
• Application Notes: WB: Approx. 38 kDa band observed in lysates of MCF7 and of human, mouse, rat and porcine heart (calculated MW of 41.5 kDa band according to NP_777358.1).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for IDH3G Antibody

• EC 1.1.1
• EC 1.1.1.41
• H-IDHG
• IDH-gamma
• isocitrate dehydrogenase 3 (NAD+) gamma
• isocitrate dehydrogenase, NAD(+)-specific, mitochondrial, gamma subunit
• mitochondrial
• NAD (H)-specific isocitrate dehydrogenase gamma subunit
• NAD+-specific ICDH

Background

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein encoded by this gene is the gamma subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase. This gene is a candidate gene for periventricular heterotopia. Several alternatively spliced transcript variants of this gene have been described, but only some of their full length natures have been determined. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IDH3G Antibody (NBP1-78786)(1)

Publication using NBP1-78786

• Bzymek KP, Colman RF. Role of alpha-Asp181, beta-Asp192, and gamma-Asp190 in the distinctive subunits of human NAD-specific isocitrate dehydrogenase. Biochemistry 2007-05-08 [PMID: 17432878]

Bioinformatics

• Gene Symbol: IDH3G
• Entrez:
  • 3421(Human)
  • 15929(Mouse)
  • 25179(Rat)
• Uniprot:
  • NP_004126.1(Human)