| Reactivity | VSpecies Glossary |
| Applications | ELISA |
| Clone | 610 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | CoraFluor 1 |
| Description | CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(TM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
CoraFluor(TM) 1, amine reactive CoraFluor(TM) 1, thiol reactive For more information, please see our CoraFluor(TM) TR-FRET technology flyer. |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | This product is a recombinant monoclonal antibody expressed from HEK293 cells. |
| Specificity | Recognizes the Spike of HCoV-NL63 in ELISA. Doesn't recognize the Spike of SARS-CoV-2, SARS-CoV, MERS-CoV, HCoV-229E, HCoV-HKU1 (isolate N5), HCoV-OC43. Has cross-reactivity in ELISA with: HCoV-NL63 Spike S1+S2 ECD-His No cross-reactivity in ELISA and WB with: SARS-CoV-2 Spike S1+S2 ECD-His SARS-CoV-2 Spike S2 ECD-His SARS-CoV-2 Spike S1-His SARS-CoV-2 Spike S1 NTD-Fc & AVI SARS-CoV-2 Spike S1 NTD-His & AVI SARS-CoV-2 Spike RBD-His SARS-CoV Spike S1+S2 ECD-His MERS-CoV Spike S1+S2 ECD-His HCoV-HKU1 (isolate N5) Spike S1+S2 ECD-His HCoV-229E Spike S1+S2 ECD-His HCoV-OC43 Spike S1+S2 ECD-His No cross-reactivity in WB with: HCoV-NL63 Spike S1+S2 ECD-His |
| Source | HEK293 |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | S |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Application Notes | Optimal dilution of this antibody should be experimentally determined. |
| Storage | Store at 4C in the dark. Do not freeze. |
| Buffer | PBS |
| Preservative | No Preservative |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | S |
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")