HspBAP1 Antibody

Western Blot: HspBAP1 Antibody [NBP1-92014] - Analysis in human testis tissue.

Immunocytochemistry/Immunofluorescence: HspBAP1 Antibody [NBP1-92014] - Staining of human cell line SK-MEL-30 shows localization to nucleus & mitochondria.

Immunohistochemistry-Paraffin: HspBAP1 Antibody [NBP1-92014] - Staining of human stomach shows strong cytoplasmic positivity in glandular cells.

Product Discontinued

HspBAP1 Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: LQQPAIFCNMVFDWPARHWNAKYLSQVLHGKQIRFRMGMKSMSTVPQFETTCNYVEATLEEFLTWNCDQSSISGPFRDYDHSKFWAYADYKYFVSLFEDKTDLFQDVKWSDFGFPGRNGQESTLWIGSLGAHTPCHLDSYGCNLVFQ
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HSPBAP1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1 - 4 ug/ml
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. Use in ELISA reported in scientific literature (PMID 29310668).

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (86%), Rat (86%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for HspBAP1 Antibody

• FLJ22623
• heat shock 27 kDa associated protein
• HSPB (heat shock 27kD) associated protein 1
• HSPB (heat shock 27kDa) associated protein 1
• HSPB1-associated protein 1,27 kDa heat shock protein-associated protein 1
• PASS1FLJ39386
• Protein associated with small stress protein 1
• protein associating with small stress protein PASS1

Background

HSPBAP1 may play a role in cellular stress response

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HspBAP1 Antibody (NBP1-92014)(1)

Publication using NBP1-92014

• Brescia AC, Simonds MM, McCahan SM et al. Prior to extension, Transcriptomes of fibroblast-like Synoviocytes from extended and Polyarticular juvenile idiopathic arthritis are indistinguishable. Pediatr Rheumatol Online J 2018-01-08 [PMID: 29310668] (ELISA, Human)

Bioinformatics

• Gene Symbol: HSPBAP1