Western Blot: HspBAP1 Antibody [NBP1-92014] - Analysis in control (vector only transfected HEK293T lysate) and HSPBAP1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T ...read more
Immunocytochemistry/ Immunofluorescence: HspBAP1 Antibody [NBP1-92014] - Staining of human cell line SK-MEL-30 shows localization to nucleus & mitochondria.
Immunohistochemistry-Paraffin: HspBAP1 Antibody [NBP1-92014] - Staining of human stomach shows strong cytoplasmic positivity in glandular cells.
Immunocytochemistry/ Immunofluorescence: HspBAP1 Antibody [NBP1-92014] - Staining of human cell line U-2 OS shows localization to mitochondria.
This antibody was developed against Recombinant Protein corresponding to amino acids: LQQPAIFCNMVFDWPARHWNAKYLSQVLHGKQIRFRMGMKSMSTVPQFETTCNYVEATLEEFLTWNCDQSSISGPFRDYDHSKFWAYADYKYFVSLFEDKTDLFQDVKWSDFGFPGRNGQESTLWIGSLGAHTPCHLDSYGCNLVFQ
Specificity of human HspBAP1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Immunogen affinity purified
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For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100. Use in ELISA reported in scientific literature (PMID 29310668).
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