HKDC1 Antibody

Immunocytochemistry/Immunofluorescence: HKDC1 Antibody [NBP2-76936] - ICC staining HKDC1 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry: HKDC1 Antibody [NBP2-76936] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-HKDC1 antibody. Counter stained with hematoxylin.

Flow Cytometry: HKDC1 Antibody [NBP2-76936] - Flow cytometric analysis of LOVO cells with HKDC1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; green). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: HKDC1 Antibody [NBP2-76936] - ICC staining HKDC1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: HKDC1 Antibody [NBP2-76936] - ICC staining HKDC1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry: HKDC1 Antibody [NBP2-76936] - Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-HKDC1 antibody. Counter stained with hematoxylin.

Product Discontinued

HKDC1 Antibody Summary

• Immunogen: Recombinant protein within Human HKDC1 aa 266-462 / 917. (SwissProt: Q2TB90 Human; SwissProt: Q91W97 Mouse)
• Localization: Cytosol. Mitochondrion.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HKDC1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500
• Theoretical MW: 90 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.2% BSA, 50% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for HKDC1 Antibody

• EC 2.7.1
• EC 2.7.1.1
• FLJ22761
• FLJ37767
• hexokinase domain containing 1
• Hexokinase domain-containing protein 1
• MGC125688
• putative hexokinase HKDC1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HKDC1