Histone H3.3B Recombinant Protein Antigen

• Reactivity: Hu
• Applications: AC

Histone H3.3B Recombinant Protein Antigen Summary

• Description: A recombinant protein antigen with a N-terminal His6-ABP tag corresponding to human Histone H3.3B
  
  

  Source: E.coli

  Amino Acid Sequence: KPHRYRPGTVALREIRRYQKSTELLIR

  Fusion Tag: N-terminal His6ABP (ABP = Albumin Binding Protein derived from Streptococcal Protein G)

• Protein/Peptide Type: Recombinant Protein Antigen
• Gene: H3-3B
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

• Dilutions:
  • Antibody Competition 10-100 molar excess
• Application Notes: This recombinant antigen is only intended to be used as a blocking agent to confirm antibody specificity with the corresponding antibody, catalog number NBP3-24899It is purified by IMAC chromatography, and the expected concentration is greater than 0.5 mg/ml.For current lot information, including availability, please contact our technical support team click nb-technical@bio-techne.com.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS and 1M Urea, pH 7.4.
• Preservative: No Preservative
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Alternate Names for Histone H3.3B Recombinant Protein Antigen

• H3 histone, family 3A
• H3 histone, family 3B (H3.3B)
• H3.3BH3.3A
• H3F3
• H3F3A
• histone H3.3

Background

Histone H3.3B is 136 amino acids long, weighs approximately 15 kDa, and is part of a group of basic nuclear proteins called histones, which are responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Current studies are being done on several diseases and disorders including systemic lupus erythematosus, lupus erythematosus, malaria, leukemia, retinoblastoma, and immunodeficiency. Histone H3.3B has also been shown to have interactions with PELP1, HIST1H4A, HIST1H4B, HIST1H4C, and HIST1H4D in pathways such as the histone modification, development notch signaling, signal transduction for Activin A, amyloids, hemostasis, meiosis, meiotic recombination, and systemic lupus erythematosus pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: H3-3B